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Polymers 2016, 8(8), 279; doi:10.3390/polym8080279

Rheological Properties of DNA Molecules in Solution: Molecular Weight and Entanglement Influences

1
Laboratoire Rhéologie et Procédés (LRP), University Grenoble Alpes, Grenoble F-38000, France
2
Centre National de la Recherche Scientifique (CNRS), Laboratoire Rhéologie et Procédés (LRP), Grenoble F-38000, France
3
Departamento de Ingeniería Química, Universidad de Guadalajara, Blvd. M. García Barragán, Guadalajara C.P. 44430, Mexico
4
Biomaterials applications, 6 rue Lesdiguières, Grenoble F-38000, France
*
Author to whom correspondence should be addressed.
Academic Editor: Christine Wandrey
Received: 17 June 2016 / Revised: 25 July 2016 / Accepted: 29 July 2016 / Published: 3 August 2016
(This article belongs to the Collection Polyelectrolytes)
View Full-Text   |   Download PDF [434 KB, uploaded 3 August 2016]   |  

Abstract

Molecular weight, stiffness, temperature, and polymer and ionic concentrations are known to widely influence the viscosity of polymer solutions. Additionally, polymer molecular weight—which is related to its dimensions in solution—is one of its most important characteristics. In this communication, low molecular weight DNA from salmon sperm was purified and then studied in solutions in a wide concentration range (between 0.5 and 1600 mg/mL). The intrinsic viscosity of this low molecular weight DNA sample was firstly determined and the evidence of the overlap concentration was detected around the concentration of 125 mg/mL. The chain characteristics of these short molecules were studied in terms of the influence of their molecular weight on the solution viscosities and on the overlap parameter CDNA[η]. Furthermore, to complete previously reported experimental data, solutions of a large molecular weight DNA from calf-thymus were studied in a high concentration range (up to 40 mg/mL). The rheological behavior is discussed in terms of the generalized master curve obtained from the variation of the specific viscosity at zero shear rate (ηsp,0) as a function of CDNA[η]. View Full-Text
Keywords: low and high molecular weight DNA; hydrodynamic behavior; overlap parameter CDNA[η] low and high molecular weight DNA; hydrodynamic behavior; overlap parameter CDNA[η]
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Bravo-Anaya, L.M.; Pignon, F.; Martínez, F.A.S.; Rinaudo, M. Rheological Properties of DNA Molecules in Solution: Molecular Weight and Entanglement Influences. Polymers 2016, 8, 279.

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