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Polymers 2016, 8(6), 221; doi:10.3390/polym8060221

Production and Characterization of a Novel, Electrospun, Tri-Layer Polycaprolactone Membrane for the Segregated Co-Culture of Bone and Soft Tissue

1
Faculty of Dentistry, Naresuan University, Phitsanulok 65000, Thailand
2
Kroto Research Institute, University of Sheffield, Sheffield S3 7HQ, UK
3
INSIGNEO Institute for in silico Medicine, University of Sheffield, Sheffield S10 2TN, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Jianxun Ding
Received: 12 May 2016 / Revised: 31 May 2016 / Accepted: 1 June 2016 / Published: 7 June 2016
(This article belongs to the Special Issue Functional Polymers for Medical Applications)
View Full-Text   |   Download PDF [3711 KB, uploaded 7 June 2016]   |  

Abstract

Composite tissue-engineered constructs combining bone and soft tissue have applications in regenerative medicine, particularly dentistry. This study generated a tri-layer, electrospun, poly-ε-caprolactone membrane, with two microfiber layers separated by a layer of nanofibers, for the spatially segregated culture of mesenchymal progenitor cells (MPCs) and fibroblasts. The two cell types were seeded on either side, and cell proliferation and spatial organization were investigated over several weeks. Calcium deposition by MPCs was detected using xylenol orange (XO) and the separation between fibroblasts and the calcified matrix was visualized by confocal laser scanning microscopy. SEM confirmed that the scaffold consisted of two layers of micron-diameter fibers with a thin layer of nano-diameter fibers in-between. Complete separation of cell types was maintained and calcified matrix was observed on only one side of the membrane. This novel tri-layer membrane is capable of supporting the formation of a bilayer of calcified and non-calcified connective tissue. View Full-Text
Keywords: mesenchymal stem cells; maxillofacial surgery; bone tissue engineering; soft tissue engineering; scaffold; electrospinning mesenchymal stem cells; maxillofacial surgery; bone tissue engineering; soft tissue engineering; scaffold; electrospinning
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Puwanun, S.; Bye, F.J.; Ireland, M.M.; MacNeil, S.; Reilly, G.C.; Green, N.H. Production and Characterization of a Novel, Electrospun, Tri-Layer Polycaprolactone Membrane for the Segregated Co-Culture of Bone and Soft Tissue. Polymers 2016, 8, 221.

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