Next Article in Journal
Production and Characterization of a Novel, Electrospun, Tri-Layer Polycaprolactone Membrane for the Segregated Co-Culture of Bone and Soft Tissue
Next Article in Special Issue
Enzymatic Synthesis of Biobased Polyesters and Polyamides
Previous Article in Journal
Influence of Polyplex Formation on the Performance of Star-Shaped Polycationic Transfection Agents for Mammalian Cells
Previous Article in Special Issue
Papain-Catalyzed Synthesis of Polyglutamate Containing a Nylon Monomer Unit
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Polymers 2016, 8(6), 223; doi:10.3390/polym8060223

Design and Preparation of Nano-Lignin Peroxidase (NanoLiP) by Protein Block Copolymerization Approach

1
Department of Chemistry, Anadolu University, 26470 Eskisehir, Turkey
2
Karen Biotechnol Ltd., Anadolu University, Technol Pk, 26470 Eskisehir, Turkey
3
Yunus Emre Vocational School, Department of Medical Services and Techniques, Anadolu University, 26470 Eskisehir, Turkey
*
Author to whom correspondence should be addressed.
Academic Editor: Katja Loos
Received: 22 April 2016 / Revised: 9 May 2016 / Accepted: 1 June 2016 / Published: 6 June 2016
(This article belongs to the Special Issue Enzymatic Polymer Synthesis)
View Full-Text   |   Download PDF [2930 KB, uploaded 6 June 2016]   |  

Abstract

This study describes the preparation of nanoprotein particles having lignin peroxidase (LiP) using a photosensitive microemulsion polymerization technique. The protein-based nano block polymer was synthesized by cross-linking of ligninase enzyme with ruthenium-based aminoacid monomers. This type polymerization process brought stability in different reaction conditions, reusability and functionality to the protein-based nano block polymer system when compared the traditional methods. After characterization of the prepared LiP copolymer nanoparticles, enzymatic activity studies of the nanoenzymes were carried out using tetramethylbenzidine (TMB) as the substrate. The parameters such as pH, temperature and initial enzyme concentration that affect the activity, were investigated by using prepared nanoLip particles and compared to free LiP. The reusability of the nano-LiP particles was also investigated and the obtained results showed that the nano-LiP particles exhibited admirable potential as a reusable catalyst. View Full-Text
Keywords: nanoenzyme; lignin peroxidase (LiP); photosensitive monomer; ANADOLUCA; tetramethylbenzidine (TMB) nanoenzyme; lignin peroxidase (LiP); photosensitive monomer; ANADOLUCA; tetramethylbenzidine (TMB)
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Tay, T.; Köse, E.; Keçili, R.; Say, R. Design and Preparation of Nano-Lignin Peroxidase (NanoLiP) by Protein Block Copolymerization Approach. Polymers 2016, 8, 223.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Polymers EISSN 2073-4360 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top