Cancers 2012, 4(3), 808-820; doi:10.3390/cancers4030808
Article

Succinate Dehydrogenase B Subunit Immunohistochemical Expression Predicts Aggressiveness in Well Differentiated Neuroendocrine Tumors of the Ileum

1, 2, 3, 1, 4, 5, 2 and 1,6,* email
Received: 18 July 2012; in revised form: 6 August 2012 / Accepted: 9 August 2012 / Published: 16 August 2012
(This article belongs to the Special Issue Neuroendocrine Tumors)
View Full-Text   |   Download PDF [365 KB, uploaded 16 August 2012]
Abstract: Immunohistochemical loss of the succinate dehydrogenase subunit B (SDHB) has recently been reported as a surrogate biomarker of malignancy in sporadic and familial pheocromocytomas and paragangliomas through the activation of hypoxia pathways. However, data on the prevalence and the clinical implications of SDHB immunoreactivity in ileal neuroendocrine tumors are still lacking. Thirty-one consecutive, advanced primary midgut neuroendocrine tumors and related lymph node or liver metastases from 24 males and seven females were immunohistochemically assessed for SDHB. All patients were G1 tumors (Ki-67 labeling index ≤2%). SDHB immunohistochemistry results were expressed as immunostaining intensity and scored as low or strong according to the internal control represented by normal intestinal cells. Strong positivity for SDHB, with granular cytoplasmatic reactivity, was found in 77% of primary tumors (T), whilst low SDHB expression was detected in 90% of metastases (M). The combined analysis (T+M) confirmed the loss of SDHB expression in 82% of metastases compared to 18% of primary tumors. SDHB expression was inversely correlated with Ki-67 labeling index, which accounted for 1.54% in metastastic sites and 0.7% in primary tumors. A correlation between SDHB expression loss, increased Ki-67 labeling index and biological aggressiveness was shown in advanced midgut neuroendocrine tumors, suggesting a role of tumor suppressor gene.
Keywords: midgut; neuroendocrine tumors; SDHB; immunohistochemistry; Ki-67 antigen
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Milione, M.; Pusceddu, S.; Gasparini, P.; Melotti, F.; Maisonneuve, P.; Mazzaferro, V.; de Braud, F.G.; Pelosi, G. Succinate Dehydrogenase B Subunit Immunohistochemical Expression Predicts Aggressiveness in Well Differentiated Neuroendocrine Tumors of the Ileum. Cancers 2012, 4, 808-820.

AMA Style

Milione M, Pusceddu S, Gasparini P, Melotti F, Maisonneuve P, Mazzaferro V, de Braud FG, Pelosi G. Succinate Dehydrogenase B Subunit Immunohistochemical Expression Predicts Aggressiveness in Well Differentiated Neuroendocrine Tumors of the Ileum. Cancers. 2012; 4(3):808-820.

Chicago/Turabian Style

Milione, Massimo; Pusceddu, Sara; Gasparini, Patrizia; Melotti, Flavia; Maisonneuve, Patrick; Mazzaferro, Vincenzo; de Braud, Filippo G.; Pelosi, Giuseppe. 2012. "Succinate Dehydrogenase B Subunit Immunohistochemical Expression Predicts Aggressiveness in Well Differentiated Neuroendocrine Tumors of the Ileum." Cancers 4, no. 3: 808-820.



Cancers EISSN 2072-6694 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert