• Submit to Cancers Login Register
• MDPI
• Journals A-Z
• For Authors
• For Editors
• About
• Open Access Policy

• Abstract
• Full-Text PDF [486 KB]
• Full-Text XML
• Article Versions Notes

• Article Statistics
• Google Scholar
• Order Reprints

• Cite this article
• Export to BibTeX
• Export to EndNote

• [+] on DOAJ
• Baig, R
• Sanders, A
• Kayani, M
• Jiang, W
• [+] on Google Scholar
• Baig, R
• Sanders, A
• Kayani, M
• Jiang, W
• [+] on PubMed
• Baig, R
• Sanders, A
• Kayani, M
• Jiang, W

•  CiteULike
•  Facebook
•  Mendeley
•  Twitter

This article is

• freely available
• re-usable

Cancers 2012, 4(3), 658-672; doi:10.3390/cancers4030658

Article

Association of Differentiation-Related Gene-1 (DRG1) with Breast Cancer Survival and in Vitro Impact of DRG1 Suppression

Ruqia Mehmood Baig ^1,2 , Andrew J. Sanders ¹ , Mahmood Akhtar Kayani ²  and Wen G. Jiang ^1,*

¹ Metastasis and Angiogenesis Research Group, Institute of Cancer and Genetics, Cardiff University School of Medicine, Cardiff CF14 4XN, UK ² Cancer Genetics Lab, COMSATS Institute of Information Technology, Islamabad 44000, Pakistan

* Author to whom correspondence should be addressed.

Received: 6 June 2012; in revised form: 29 June 2012 / Accepted: 5 July 2012 / Published: 10 July 2012

(This article belongs to the Special Issue Breast Cancers: Pathology and Biomarkers)

Download PDF Full-Text [486 KB, uploaded 10 July 2012 13:45 CEST]

Abstract: Differentiation-related gene-1, DRG1, is a metastasis suppressor gene whose expression has been shown to be dysregulated in a number of malignancies. The current study examines the expression of DRG1 in a clinical breast cohort and its association with a number of clinical pathological factors using quantitative polymerase chain reaction. Additionally, DRG1 expression is targeted in vitro using ribozyme transgene technology to explore the function of DRG1 in two human breast cancer cell lines. Low levels of DRG1 were found in patients who developed metastasis (p = 0.036) and who died of breast cancer (p = 0.0048) compared to disease free patients. Knockdown of DRG1 also resulted in significantly increased invasion and motility, but decreased matrix-adhesion in MCF7 cells. Knockdown of DRG1 seemed to have minimal impact on the cellular functions of the MDA-MB-231 breast cancer cell line causing no significant differences in cell growth, invasion, motility or matrix-adhesion. Thus, DRG1 appears to be linked to development of metastasis and death in patients who died as a result of breast cancer and may be useful as a prognostic factor as its knockdown appears to be linked with increased invasion and motility and decreased adhesion in MCF7 breast cancer cells.

Keywords: DRG1; breast cancer; metastasis; prognosis

Article Statistics

Cite This Article