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Cancers 2011, 3(4), 3909-3920; doi:10.3390/cancers3043909
Article

Forced Expression of ZNF143 Restrains Cancer Cell Growth

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Received: 9 August 2011; in revised form: 10 October 2011 / Accepted: 12 October 2011 / Published: 19 October 2011
(This article belongs to the Special Issue Prostate Cancer)
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Abstract: We previously reported that the transcription factor Zinc Finger Protein 143 (ZNF143) regulates the expression of genes associated with cell cycle and cell division, and that downregulation of ZNF143 induces cell cycle arrest at G2/M. To assess the function of ZNF143 expression in the cell cycle, we established two cells with forced expression of ZNF143 derived from PC3 prostate cancer cell lines. These cell lines overexpress genes associated with cell cycle and cell division, such as polo-like kinase 1 (PLK1), aurora kinase B (AURKB) and some minichromosome maintenance complex components (MCM). However, the doubling time of cells with forced expression of ZNF143 was approximately twice as long as its control counterpart cell line. Analysis following serum starvation and re-seeding showed that PC3 cells were synchronized at G1 in the cell cycle. Also, ZNF143 expression fluctuated, and was at its lowest level in G2/M. However, PC3 cells with forced expression of ZNF143 synchronized at G2/M, and showed lack of cell cycle-dependent fluctuation of nuclear expression of MCM proteins. Furthermore, G2/M population of both cisplatin-resistant PCDP6 cells over-expressing ZNF143 (derived from PC3 cells) and cells with forced expression of ZNF143 was significantly higher than that of each counterpart, and the doubling time of PCDP6 cells is about 2.5 times longer than that of PC3 cells. These data suggested that fluctuations in ZNF143 expression are required both for gene expression associated with cell cycle and for cell division.
Keywords: ZNF143; cell cycle; cell division ZNF143; cell cycle; cell division
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Izumi, H.; Yasuniwa, Y.; Akiyama, M.; Yamaguchi, T.; Kuma, A.; Kitamura, N.; Kohno, K. Forced Expression of ZNF143 Restrains Cancer Cell Growth. Cancers 2011, 3, 3909-3920.

AMA Style

Izumi H, Yasuniwa Y, Akiyama M, Yamaguchi T, Kuma A, Kitamura N, Kohno K. Forced Expression of ZNF143 Restrains Cancer Cell Growth. Cancers. 2011; 3(4):3909-3920.

Chicago/Turabian Style

Izumi, Hiroto; Yasuniwa, Yoshihiro; Akiyama, Masaki; Yamaguchi, Takahiro; Kuma, Akihiro; Kitamura, Noriaki; Kohno, Kimitoshi. 2011. "Forced Expression of ZNF143 Restrains Cancer Cell Growth." Cancers 3, no. 4: 3909-3920.


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