Cancers 2011, 3(4), 3824-3837; doi:10.3390/cancers3043824
Article

The Expression of MTUS1/ATIP and Its Major Isoforms, ATIP1 and ATIP3, in Human Prostate Cancer

* email, email, email, email, email and email
Received: 5 August 2011; in revised form: 22 September 2011 / Accepted: 28 September 2011 / Published: 11 October 2011
(This article belongs to the Special Issue Prostate Cancer)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Angiotensin II (Ang II), the main effector of the renin angiotensin system, acts upon two distinct transmembrane receptors, the Ang II type 1 and the type 2 (AT2-) receptor, to induce promotion and inhibition of ERK2 phosphorylation. The AT2-receptor, through an interaction with its putative signaling partner MTUS1/ATIP (AT2-receptor interacting protein), inhibits the mitogenic effects of EGF in prostate cancer cell lines representing both early and late stage disease. This is the first report on the expression of ATIP in normal and malignant human prostatic biopsies. The expression of ATIP and its major isoforms, ATIP1 and ATIP3, in normal prostatic cells and three prostate cancer cell lines was examined using QPCR and immunohistochemistry. Human biopsies containing benign prostatic hyperplasia (BPH), high grade prostatic intraepithelial neoplasia (HGPIN) and well, moderately and poorly differentiated prostate cancer were also examined. Overall, ATIP1 and ATIP3 mRNA expression was increased in malignant compared to normal tissues and cell lines. ATIP immunostaining was low or absent in both the basal and columnar epithelial cell layers surrounding BPH acini; however, it was observed in high concentration in neoplastic epithelial cells of HGPIN and was clearly evident in cytoplasms of malignant cells in all prostate cancer grades. ATIP immunostaining was also identified in the cytoplasms of LNCaP and PC3 prostate cancer cells. As the AT2-receptor/ATIP inhibitory signaling pathway exists in malignant cells in all grades of prostate cancer, enhancement of this pathway may be a therapeutic target even after the development of androgen-independence.
Keywords: Angiotensin II (Ang II); Ang II type 2 (AT2-) receptor; AT2-receptor interacting protein (ATIP); prostate cancer; high grade prostatic intraepithelial neoplasia (HGPIN)
PDF Full-text Download PDF Full-Text [1184 KB, uploaded 11 October 2011 18:00 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Louis, S.N.; Chow, L.T.; Varghayee, N.; Rezmann, L.A.; Frauman, A.G.; Louis, W.J. The Expression of MTUS1/ATIP and Its Major Isoforms, ATIP1 and ATIP3, in Human Prostate Cancer. Cancers 2011, 3, 3824-3837.

AMA Style

Louis SN, Chow LT, Varghayee N, Rezmann LA, Frauman AG, Louis WJ. The Expression of MTUS1/ATIP and Its Major Isoforms, ATIP1 and ATIP3, in Human Prostate Cancer. Cancers. 2011; 3(4):3824-3837.

Chicago/Turabian Style

Louis, Simon N.S.; Chow, Laurie T.C.; Varghayee, Naghmeh; Rezmann, Linda A.; Frauman, Albert G.; Louis, William J. 2011. "The Expression of MTUS1/ATIP and Its Major Isoforms, ATIP1 and ATIP3, in Human Prostate Cancer." Cancers 3, no. 4: 3824-3837.

Cancers EISSN 2072-6694 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert