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Screening Technologies for Target Identification in Pancreatic Cancer
Department of Gastroenterology and Endocrinology, University Hospital, Philipps-University Marburg, Baldinger Strasse, D-35043 Marburg, Germany
* Author to whom correspondence should be addressed.
Received: 23 November 2010; in revised form: 21 December 2010 / Accepted: 23 December 2010 / Published: 29 December 2010
Abstract: Pancreatic cancer exhibits an extraordinarily high level of resistance to almost any kind of systemic therapy evaluated in clinical trials so far. Therefore, the identification of novel therapeutic targets is urgently required. High-throughput screens have emerged as an important tool to identify putative targets for diagnosis and therapy in an unbiased manner. More than a decade ago, microarray technology was introduced to identify differentially expressed genes in pancreatic cancer as compared to normal pancreas, chronic pancreatitis and other cancer types located in close proximity to the pancreas. In addition, proteomic screens have facilitated the identification of differentially secreted proteins in body fluids of pancreatic cancer patients, serving as possible biomarkers. Recently, RNA interference-based loss-of-function screens have been used to identify functionally relevant genes, whose knock-down has impact on pancreatic cancer cell viability, thereby representing potential new targets for therapeutic intervention. This review summarizes recent results of transcriptional, proteomic and functional screens in pancreatic cancer and discusses potentials and limitations of the respective technologies as well as their impact on future therapeutic developments.
Keywords: pancreatic cancer; high-throughput screen; microarray; RNA interference; loss-of-function screen
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MDPI and ACS Style
Michl, P.; Ripka, S.; Gress, T.; Buchholz, M. Screening Technologies for Target Identification in Pancreatic Cancer. Cancers 2011, 3, 79-90.
Michl P, Ripka S, Gress T, Buchholz M. Screening Technologies for Target Identification in Pancreatic Cancer. Cancers. 2011; 3(1):79-90.
Michl, Patrick; Ripka, Stefanie; Gress, Thomas; Buchholz, Malte. 2011. "Screening Technologies for Target Identification in Pancreatic Cancer." Cancers 3, no. 1: 79-90.