Cancers 2011, 3(1), 91-105; doi:10.3390/cancers3010091
Article

Detection of up to 65% of Precancerous Lesions of the Human Colon and Rectum by Mutation Analysis of APC, K-Ras, B-Raf and CTNNB1

1,†email, 1,†,* email, 2email, 3email, 4email, 4email and 1,5,* email
Received: 24 October 2010; in revised form: 7 December 2010 / Accepted: 20 December 2010 / Published: 29 December 2010
(This article belongs to the Special Issue Prognostic and Predictive Factors in Colorectal Cancer)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In the present study a recently conceived 4-gene marker panel covering the Wnt and Ras-Raf-MEK-MAPK signaling pathways was used to analyze 20 colorectal serrated lesions and 41 colorectal adenoma samples and to determine the percentage of each of the above-mentioned potentially precancerous lesions carrying at least one of the four above-mentioned genes in a mutated form. CTNNB1 and B-Raf were screened by PCR-single-strand conformation polymorphism analysis, K-Ras by restriction fragment length polymorphism analysis and the APC gene mutation cluster region (codons 1243–1567) by direct DNA sequencing. APC mutations were only detected in 10% of the serrated lesions but in 34% of the adenomas. Twenty percent of the serrated lesions and 14% of the adenomas carried a mutated K-Ras. B-Raf was found to be mutated in 50% of the serrated lesions and in 22% of the adenomas. CTNNB1 was altered in 12% of the adenomas, but not in serrated lesions. By using the above gene marker panel it could be shown that 65% of the serrated lesions and 61% of the adenomas carried at least one of the four genes in a mutated form. Based on its excellent performance in detecting mutations in sporadic preneoplastic (in this study) and neoplastic lesions (in a previous study) of the human colon and rectum, this primer combination might also be suited to efficiently and non-invasively detect genetic alterations in stool DNA of patients with early colorectal cancer.
Keywords: adenomas; APC; B-Raf; CTNNB1; gene mutations; human colon; serrated lesions; K-Ras; primer panel
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MDPI and ACS Style

Schneider, M.; Scholtka, B.; Gottschalk, U.; Faiss, S.; Schatz, D.; Berghof-Jäger, K.; Steinberg, P. Detection of up to 65% of Precancerous Lesions of the Human Colon and Rectum by Mutation Analysis of APC, K-Ras, B-Raf and CTNNB1. Cancers 2011, 3, 91-105.

AMA Style

Schneider M, Scholtka B, Gottschalk U, Faiss S, Schatz D, Berghof-Jäger K, Steinberg P. Detection of up to 65% of Precancerous Lesions of the Human Colon and Rectum by Mutation Analysis of APC, K-Ras, B-Raf and CTNNB1. Cancers. 2011; 3(1):91-105.

Chicago/Turabian Style

Schneider, Mandy; Scholtka, Bettina; Gottschalk, Uwe; Faiss, Siegbert; Schatz, Daniela; Berghof-Jäger, Kornelia; Steinberg, Pablo. 2011. "Detection of up to 65% of Precancerous Lesions of the Human Colon and Rectum by Mutation Analysis of APC, K-Ras, B-Raf and CTNNB1." Cancers 3, no. 1: 91-105.

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