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A Comparative Study of Two Folate-Conjugated Gold Nanoparticles for Cancer Nanotechnology Applications
Department of Bioengineering, University of Illinois at Chicago, 851 S. Morgan St. (MC 063), Chicago, IL 60607, USA
Department of Medical Physics, Tehran University of Medical Sciences, Tehran, Iran
* Author to whom correspondence should be addressed.
Received: 29 October 2010; in revised form: 10 November 2010 / Accepted: 11 November 2010 / Published: 18 November 2010
Abstract: We report a comparative study of synthesis, characteristics and in vitro tests of two folate-conjugated gold nanoparticles (AuNP) differing in linkers and AuNP sizes for selective targeting of folate-receptor positive cancerous cells. The linkers chosen were 4-aminothiophenol (4Atp) and 6-mercapto-1-hexanol (MH) with nanoconjugate products named Folate-4Atp-AuNP and Folate-MH-AuNP. We report the folate-receptor tissue distribution and its endocytosis for targeted nanotechnology. Comparison of the two nanoconjugates’ syntheses and characterization is also reported, including materials and methods of synthesis, UV-visible absorption spectroscopic measurements, Fourier Transform Infra Red (FTIR) measurements, Transmission electron microscopy (TEM) images and size distributions, X-ray diffraction data, elemental analyses and chemical stability comparison. In addition to the analytical characterization of the nanoconjugates, the cell lethality was measured in HeLa (high level of folate receptor expression) and MCF-7 (low level of folate receptor expression) cells. The nanoconjugates themselves, as well as the intense pulsed light (IPL) were not harmful to cell viability. However, upon stimulation of the folate targeted nanoconjugates with the IPL, ~98% cell killing was found in HeLa cells and only ~9% in MCF-7 cells after four hours incubation with the nanoconjugate. This demonstrates that folate targeting is effective in selecting for specific cell populations. Considering the various comparisons made, we conclude that Folate-4Atp-AuNP is superior to Folate-MH-AuNP for cancer therapy.
Keywords: 4-aminothiophenol; 6-mercapto-1-hexanol; cancer nanotechnology; folate; folate receptor; folic acid; gold nanoparticle; nanoconjugate; photothermal treatment
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MDPI and ACS Style
Mansoori, G.A.; Brandenburg, K.S.; Shakeri-Zadeh, A. A Comparative Study of Two Folate-Conjugated Gold Nanoparticles for Cancer Nanotechnology Applications. Cancers 2010, 2, 1911-1928.
Mansoori GA, Brandenburg KS, Shakeri-Zadeh A. A Comparative Study of Two Folate-Conjugated Gold Nanoparticles for Cancer Nanotechnology Applications. Cancers. 2010; 2(4):1911-1928.
Mansoori, G. Ali; Brandenburg, Kenneth S.; Shakeri-Zadeh, Ali. 2010. "A Comparative Study of Two Folate-Conjugated Gold Nanoparticles for Cancer Nanotechnology Applications." Cancers 2, no. 4: 1911-1928.