A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plasmid Construction and Generation of E. Coli Strains
2.2. Cell Culture
2.3. Apoptosis Experiments
2.4. Purified Violacein Experiments
2.5. Microfluidic Chip Fabrication
2.6. Surfactant and Oil System
2.7. Microfluidic Set-Up
2.8. Preparation of Droplet Samples for FACS Analysis
2.9. Preparation of Samples for COPAS Sorting
3. Results
3.1. Strategy
3.2. Extraction-Free Method for Mammalian Cell Delivery of Microbially Produced Natural Products
3.3. Droplet Microfluidic Workflow
3.4. Cell Aggregates for Efficient Screening of Pro-Apoptotic Compounds
4. Discussion
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Theodorou, E.; Scanga, R.; Twardowski, M.; Snyder, M.P.; Brouzes, E. A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds. Micromachines 2017, 8, 230. https://doi.org/10.3390/mi8080230
Theodorou E, Scanga R, Twardowski M, Snyder MP, Brouzes E. A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds. Micromachines. 2017; 8(8):230. https://doi.org/10.3390/mi8080230
Chicago/Turabian StyleTheodorou, Elias, Randall Scanga, Mariusz Twardowski, Michael P. Snyder, and Eric Brouzes. 2017. "A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds" Micromachines 8, no. 8: 230. https://doi.org/10.3390/mi8080230