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Micromachines 2017, 8(3), 64;

Observation Interface of PDMS Membrane in a Microfluidic Chip Based on One-Step Molding

Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230026, China
National Synchrotron Radiation Laboratory, Unitersity of Science and Technology of China, Hefei 230029, China
Institute of Materials, China Academy of Engineering Physics, Mianyang 621700, China
Authors to whom correspondence should be addressed.
Academic Editors: Adam Abate and Leqian Liu
Received: 26 December 2016 / Revised: 6 February 2017 / Accepted: 15 February 2017 / Published: 23 February 2017
(This article belongs to the Special Issue Enabling Microfluidic Technologies for Single Cell Analysis)
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Nowadays, researchers are focusing on sorting, characterizing and detecting micron or submicron particles or bacteria in microfluidic chips. However, some contradictions hinder the applications of conventional microfluidic chips, including the low working distance of high resolving power microscopy and the low light transmittance of conventional microfluidic chips. In this paper, a rapid and readily accessible microfluidic fabrication method is presented to realize observation with high magnification microscopy. With the one-step molding process, the interconnections, the thin observation interface of polydimethylsiloxane (PDMS) membrane and microfluidic channels were integrated into an intact PDMS replica. Three kinds of PDMS replicas with different auxiliary beams were designed and optimized by leakage experiments and analytical software. The observation interfaces of a 170 μm thickness PDMS membrane enlarges the application domain of microfluidic chips. By adopting a solution of high magnification observation, microfluidic devices could be applied widely in medical science, biology and material science. View Full-Text
Keywords: microfluidic chip; polydimethylsiloxane (PDMS); PDMS membrane; imaging microfluidic chip; polydimethylsiloxane (PDMS); PDMS membrane; imaging

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Chen, X.; Hou, S.; Chu, J.; Xiong, Y.; Xiong, P.; Liu, G.; Tian, Y. Observation Interface of PDMS Membrane in a Microfluidic Chip Based on One-Step Molding. Micromachines 2017, 8, 64.

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