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Micromachines 2017, 8(8), 231; doi:10.3390/mi8080231

Digital PCR: Endless Frontier of ‘Divide and Conquer’

Beijing Advanced Innovation Center for Genomics (ICG), Biodynamic Optical Imaging Center (BIOPIC), College of Engineering, School of Life Sciences, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
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Received: 28 June 2017 / Revised: 18 July 2017 / Accepted: 18 July 2017 / Published: 25 July 2017
(This article belongs to the Special Issue Insights and Advancements in Microfluidics)
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Abstract

Digital polymerase chain reaction (PCR) is becoming ever more recognized amid the overwhelming revolution in DNA quantification, genomics, genetics, and diagnostics led by technologies such as next generation sequencing and studies at the single-cell level. The demand to quantify the amount of DNA and RNA has been driven to the molecular level and digital PCR, with its unprecedented quantification capability, is sure to shine in the coming era. Two decades ago, it emerged as a concept; yet one decade ago, integration with microfluidics invigorated this field. Today, many methods have come to public knowledge and applications surrounding digital PCR is mounting. However, to reach wider accessibility and better practicality, efforts are needed to tackle the remaining problems. This perspective looks back at several inspiring and influential digital PCR approaches in the past and tries to provide a futuristic picture of the trends of digital PCR technologies to come. View Full-Text
Keywords: digital polymerase chain reaction (PCR); microfluidics; emulsion droplet; microwell chip digital polymerase chain reaction (PCR); microfluidics; emulsion droplet; microwell chip
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Liao, P.; Huang, Y. Digital PCR: Endless Frontier of ‘Divide and Conquer’. Micromachines 2017, 8, 231.

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