Next Article in Journal
Design and Fabrication of a 2-Axis Electrothermal MEMS Micro-Scanner for Optical Coherence Tomography
Next Article in Special Issue
Ink-Jet Printing of Micro-Electro-Mechanical Systems (MEMS)
Previous Article in Journal
Editorial for the Special Issue on Micro/Nano-Chip Electrokinetics
Previous Article in Special Issue
The Emerging Frontiers and Applications of High-Resolution 3D Printing
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Micromachines 2017, 8(5), 147; doi:10.3390/mi8050147

3D Cardiac Cell Culture on Nanofiber Bundle Substrates for the Investigation of Cell Morphology and Contraction

Tsinghua National Laboratory for Information Science and Technology, Institute of Microelectronics, Tsinghua University, Beijing 100084, China
*
Author to whom correspondence should be addressed.
Academic Editors: Chee Kai Chua and Wai Yee Yeong
Received: 31 March 2017 / Revised: 24 April 2017 / Accepted: 1 May 2017 / Published: 5 May 2017
(This article belongs to the Special Issue Bioprinting and 3D Printing in MEMS Technology)
View Full-Text   |   Download PDF [13001 KB, uploaded 5 May 2017]   |  

Abstract

Cardiac failure is a quite severe condition that can result in life-threatening consequences. Cardiac tissue engineering is thought to be one of the most promising technologies to reconstruct damaged cardiac muscles and facilitate myocardial tissue regeneration. We report a new nanofiber bundle substrate for three-dimensional (3D) cardiac cell culture as a platform to investigate cell morphology and contraction. Polymeric nanofiber bundles with various patterns act as physical cues to align the cardiac cell sheets. Comparing the uniaxial alignment with the randomly distributed pattern, we found that the bundles with the former pattern have more “grooves” for the settlement of cardiomyocytes in a 3D structure than the latter. The cardiomyocytes loaded on the aligned nanofiber bundles tend to grow along the fiber axis. The interfacial structure between a single cardiomyocyte in the cardiac cell sheet and the attached nanofibers was observed using environmental scanning electron microscope. Immunofluorescence imaging showed that the uniaxially aligned nanofibers greatly promoted cell attachment and alignment of the cardiomyocytes because of the matching morphology between the nanofiber pattern and the biological components. Moreover, we concluded that the aligned polymeric nanofibers could be a promising substrate suitable for the anisotropic contraction of cardiac cell sheets. View Full-Text
Keywords: three-dimensional cell culture; cardiomyocyte; nanofiber bundles; nanofiber pattern; cell morphology; cell contraction three-dimensional cell culture; cardiomyocyte; nanofiber bundles; nanofiber pattern; cell morphology; cell contraction
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Liu, X.; Xu, S.; Kuang, X.; Wang, X. 3D Cardiac Cell Culture on Nanofiber Bundle Substrates for the Investigation of Cell Morphology and Contraction. Micromachines 2017, 8, 147.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top