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Micromachines 2016, 7(6), 96; doi:10.3390/mi7060096

Application of Vertical Electrodes in Microfluidic Channels for Impedance Analysis

Laboratory of Biosensing and MicroMechatronics, School of Materials Science and Engineering, Southwest Jiaotong University, 610031 Chengdu, China
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Author to whom correspondence should be addressed.
Academic Editor: Abel Martin Gonzalez Oliva
Received: 20 January 2016 / Revised: 14 March 2016 / Accepted: 17 March 2016 / Published: 25 May 2016
(This article belongs to the Special Issue Advances in Microfluidic Devices for Cell Handling and Analysis)
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Abstract

This paper presents a microfluidic device with electroplated vertical electrodes in the side walls for impedance measurement. Based on the proposed device, the impedance of NaCl solutions with different concentrations and polystyrene microspheres with different sizes was measured and analyzed. The electroplating and SU-8-PDMS (SU-8-poly(dimethylsiloxane)) bonding technologies were firstly integrated for the fabrication of the proposed microfluidic device, resulting in a tightly three-dimensional structure for practical application. The magnitude of impedance of the tested solutions in the frequency range of 1 Hz to 100 kHz was analyzed by the Zennium electrochemical workstation. The results show that the newly designed microfluidic device has potential for impedance analysis with the advantages of ease of fabrication and the integration of 3D electrodes in the side walls. The newly designed impedance sensor can distinguish different concentrations of polystyrene microspheres and may have potential for cell counting in biological areas. By integrating with other techniques such as dielectrophoresis (DEP) and biological recognition technology, the proposed device may have potential for the assay to identify foodborne pathogen bacteria. View Full-Text
Keywords: microfluidic; vertical electrodes; impedance; SU-8-PDMS bonding microfluidic; vertical electrodes; impedance; SU-8-PDMS bonding
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Li, Q.; Yuan, Y.J. Application of Vertical Electrodes in Microfluidic Channels for Impedance Analysis. Micromachines 2016, 7, 96.

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