Next Article in Journal
Farewell to Animal Testing: Innovations on Human Intestinal Microphysiological Systems
Next Article in Special Issue
Gravity-Based Precise Cell Manipulation System Enhanced by In-Phase Mechanism
Previous Article in Journal
A Robot-Assisted Cell Manipulation System with an Adaptive Visual Servoing Method
Previous Article in Special Issue
Application of Vertical Electrodes in Microfluidic Channels for Impedance Analysis
Article Menu

Export Article

Open AccessReview
Micromachines 2016, 7(7), 106; doi:10.3390/mi7070106

Cell Monitoring and Manipulation Systems (CMMSs) based on Glass Cell-Culture Chips (GC3s)

1
Leibniz Institute for Farm Animal Biology, Institute of Muscle Biology and Growth, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany
2
Department of Biology, University of Rostock, Gertrudenstr. 11a, 18057 Rostock, Germany
3
Faculty of Science and Technology, Department of Physics, Suratthani Rajabhat University, Surat Thani 84100, Thailand
4
DOT GmbH, Charles-Darwin-Ring 1A, 18059 Rostock, Germany
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Abel Martin Gonzalez Oliva
Received: 19 May 2016 / Revised: 10 June 2016 / Accepted: 20 June 2016 / Published: 24 June 2016
(This article belongs to the Special Issue Advances in Microfluidic Devices for Cell Handling and Analysis)
View Full-Text   |   Download PDF [5404 KB, uploaded 24 June 2016]   |  

Abstract

We developed different types of glass cell-culture chips (GC3s) for culturing cells for microscopic observation in open media-containing troughs or in microfluidic structures. Platinum sensor and manipulation structures were used to monitor physiological parameters and to allocate and permeabilize cells. Electro-thermal micro pumps distributed chemical compounds in the microfluidic systems. The integrated temperature sensors showed a linear, Pt1000-like behavior. Cell adhesion and proliferation were monitored using interdigitated electrode structures (IDESs). The cell-doubling times of primary murine embryonic neuronal cells (PNCs) were determined based on the IDES capacitance-peak shifts. The electrical activity of PNC networks was detected using multi-electrode arrays (MEAs). During seeding, the cells were dielectrophoretically allocated to individual MEAs to improve network structures. MEA pads with diameters of 15, 20, 25, and 35 µm were tested. After 3 weeks, the magnitudes of the determined action potentials were highest for pads of 25 µm in diameter and did not differ when the inter-pad distances were 100 or 170 µm. Using 25-µm diameter circular oxygen electrodes, the signal currents in the cell-culture media were found to range from approximately −0.08 nA (0% O2) to −2.35 nA (21% O2). It was observed that 60-nm thick silicon nitride-sensor layers were stable potentiometric pH sensors under cell-culture conditions for periods of days. Their sensitivity between pH 5 and 9 was as high as 45 mV per pH step. We concluded that sensorized GC3s are potential animal replacement systems for purposes such as toxicity pre-screening. For example, the effect of mefloquine, a medication used to treat malaria, on the electrical activity of neuronal cells was determined in this study using a GC3 system. View Full-Text
Keywords: sensorized cell-culture chip; microfluidics; AC-electrokinetics; drug screening; multi-electrode array; interdigitated electrode structure; Clark-type oxygen electrode; potentiometric pH sensor; dielectrophoresis; electroporation sensorized cell-culture chip; microfluidics; AC-electrokinetics; drug screening; multi-electrode array; interdigitated electrode structure; Clark-type oxygen electrode; potentiometric pH sensor; dielectrophoresis; electroporation
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Buehler, S.M.; Stubbe, M.; Bonk, S.M.; Nissen, M.; Titipornpun, K.; Klinkenberg, E.-D.; Baumann, W.; Gimsa, J. Cell Monitoring and Manipulation Systems (CMMSs) based on Glass Cell-Culture Chips (GC3s). Micromachines 2016, 7, 106.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top