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Micromachines 2016, 7(4), 56; doi:10.3390/mi7040056

Magnetophoretic Sorting of Single Cell-Containing Microdroplets

1
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
2
Samsung Electronics, 4 Seocho-daero 74-gil, Seocho-gu, Seoul 06620, Republic of Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Abel Martin Gonzalez Oliva
Received: 27 February 2016 / Revised: 23 March 2016 / Accepted: 28 March 2016 / Published: 30 March 2016
(This article belongs to the Special Issue Advances in Microfluidic Devices for Cell Handling and Analysis)
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Abstract

Droplet microfluidics is a promising tool for single-cell analysis since single cell can be comparted inside a tiny volume. However, droplet encapsulation of single cells still remains a challenging issue due to the low ratio of droplets containing single cells. Here, we introduce a simple and robust single cell sorting platform based on a magnetophoretic method using monodisperse magnetic nanoparticles (MNPs) and droplet microfluidics with >94% purity. There is an approximately equal amount of MNPs in the same-sized droplet, which has the same magnetic force under the magnetic field. However, the droplets containing single cells have a reduced number of MNPs, as much as the volume of the cell inside the droplet, resulting in a low magnetic force. Based on this simple principle, this platform enables the separation of single cell-encapsulated droplets from the droplets with no cells. Additionally, this device uses only a permanent magnet without any complex additional apparatus; hence, this new platform can be integrated into a single cell analysis system considering its effectiveness and convenience. View Full-Text
Keywords: magnetic nanoparticles; magnetophoresis; separation; single cell; Thalassiosira eccentrica magnetic nanoparticles; magnetophoresis; separation; single cell; Thalassiosira eccentrica
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Jo, Y.; Shen, F.; Hahn, Y.K.; Park, J.-H.; Park, J.-K. Magnetophoretic Sorting of Single Cell-Containing Microdroplets. Micromachines 2016, 7, 56.

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