Magnetophoretic Sorting of Single Cell-Containing Microdroplets
AbstractDroplet microfluidics is a promising tool for single-cell analysis since single cell can be comparted inside a tiny volume. However, droplet encapsulation of single cells still remains a challenging issue due to the low ratio of droplets containing single cells. Here, we introduce a simple and robust single cell sorting platform based on a magnetophoretic method using monodisperse magnetic nanoparticles (MNPs) and droplet microfluidics with >94% purity. There is an approximately equal amount of MNPs in the same-sized droplet, which has the same magnetic force under the magnetic field. However, the droplets containing single cells have a reduced number of MNPs, as much as the volume of the cell inside the droplet, resulting in a low magnetic force. Based on this simple principle, this platform enables the separation of single cell-encapsulated droplets from the droplets with no cells. Additionally, this device uses only a permanent magnet without any complex additional apparatus; hence, this new platform can be integrated into a single cell analysis system considering its effectiveness and convenience. View Full-Text
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Jo, Y.; Shen, F.; Hahn, Y.K.; Park, J.-H.; Park, J.-K. Magnetophoretic Sorting of Single Cell-Containing Microdroplets. Micromachines 2016, 7, 56.
Jo Y, Shen F, Hahn YK, Park J-H, Park J-K. Magnetophoretic Sorting of Single Cell-Containing Microdroplets. Micromachines. 2016; 7(4):56.Chicago/Turabian Style
Jo, Younggeun; Shen, Fengshan; Hahn, Young K.; Park, Ji-Ho; Park, Je-Kyun. 2016. "Magnetophoretic Sorting of Single Cell-Containing Microdroplets." Micromachines 7, no. 4: 56.