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Micromachines 2016, 7(3), 38; doi:10.3390/mi7030038

Allergy Testing and Drug Screening on an ITO-Coated Lab-on-a-Disc

1
Department of Electronic Engineering, Center for Advanced Research in Photonics, The Chinese University of Hong Kong, Shatin, Hong Kong, China
2
Programme of Biochemistry, School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China
3
School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China
4
Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, China
5
Institute of Chinese Medicine and State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, Hong Kong, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Nam-Trung Nguyen
Received: 7 December 2015 / Revised: 15 February 2016 / Accepted: 24 February 2016 / Published: 27 February 2016
(This article belongs to the Special Issue Centrifugal (Compact-Disc) Microfluidics for Extreme POC)
View Full-Text   |   Download PDF [4570 KB, uploaded 27 February 2016]   |  

Abstract

A lab-on-a-disc (LOAD) is a centrifugal microfluidic set-up based on centrifugal force without using micro-pumps to drive reagents and cells to various chambers through channels and valves for reactions. A LOAD coated with conductive transparent indium tin oxide (ITO) for thermal control was developed to screen allergy-blocking agents. When the acridine orange (AO)-loaded KU-812 human basophilic cells were activated in the LOAD by stimuli, AO trapped in the cytoplasmic granules was released externally as an allergic mediator mimetic to report degranulation. This response was monitored by fluorescence when the released AO in supernatant had been transferred, with a higher spinning speed, from the reaction chamber to detection chamber in the LOAD where AO reacted with exogenous DNA. We report here the principles of the system and an improved LOAD set-up with the ITO-coated glass resistive microheater to run assays at 37 °C. By using this platform, we demonstrate here for the first time that triptolide, an active ingredient from the Chinese medicine herb Tripterygium wilfordii Hook f., was able to suppress the fMLP-mediated degranulation in basophils. This serves as an example how LOADs can be used to screen agents to alleviate symptoms of allergy. View Full-Text
Keywords: acridine orange (AO); basophil activation test (BAT); bisphenol A (BPA); N-formyl-methionine-leucine-phenylalanine (fMLP); indium tin oxide (ITO); lab-on-a-disc (LOAD); protein kinase C (PKC); phorbol 12-myristate 13-acetate (PMA); Traditional Chinese Medicine (TCM); World Allergy Organization (WAO) acridine orange (AO); basophil activation test (BAT); bisphenol A (BPA); N-formyl-methionine-leucine-phenylalanine (fMLP); indium tin oxide (ITO); lab-on-a-disc (LOAD); protein kinase C (PKC); phorbol 12-myristate 13-acetate (PMA); Traditional Chinese Medicine (TCM); World Allergy Organization (WAO)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kwok, H.C.; Lau, P.M.; Wu, S.Y.; HO, H.P.; Gao, M.; Kwan, Y.W.; Wong, C.K.; Kong, S.K. Allergy Testing and Drug Screening on an ITO-Coated Lab-on-a-Disc. Micromachines 2016, 7, 38.

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