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Micromachines 2016, 7(2), 31; doi:10.3390/mi7020031

Height Resolution of Antibody Spots Measured by Spinning-Disk Interferometry on the BioCD

1
Department of Physics, University of California Berkeley, Berkeley, CA 94720-7300, USA
2
Department of Physics, Purdue University, West Lafayette, IN 47907, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Marc Madou and Nam-Trung Nguyen
Received: 3 December 2015 / Revised: 20 January 2016 / Accepted: 4 February 2016 / Published: 17 February 2016
(This article belongs to the Special Issue Centrifugal (Compact-Disc) Microfluidics for Extreme POC)
View Full-Text   |   Download PDF [5198 KB, uploaded 17 February 2016]   |  

Abstract

Spinning-disc interferometry (SDI) is a high-speed laser scanning approach to surface metrology that uses common-path interferometry to measure protein spots on a BioCD disk. The measurement sensitivity depends on the scanning pitch and on the time-base. Based on high-resolution laser scanning images of printed antibody spots, we quantify the protein sensitivity as a function of the scan parameters. For smoothly printed antibody spots scanned with a transverse spatial resolution of 1 μm, the surface height precision for a single 100 μm diameter protein spot is approximately 1 pm. This detection sensitivity sets the fundamental limit of detection for label-free BioCD biosensors performing immunoassays. View Full-Text
Keywords: differential phase contrast; common-path interferometry; silicon metrology; molecular interferometry; protein immobilization differential phase contrast; common-path interferometry; silicon metrology; molecular interferometry; protein immobilization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

O’Brien, K.; Zhao, M.; Nolte, D. Height Resolution of Antibody Spots Measured by Spinning-Disk Interferometry on the BioCD. Micromachines 2016, 7, 31.

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