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Micromachines 2016, 7(10), 181; doi:10.3390/mi7100181

Hybrid Microfluidic Platform for Multifactorial Analysis Based on Electrical Impedance, Refractometry, Optical Absorption and Fluorescence

1
CENIMAT/I3N, Department of Materials Science, Faculty of Science and Technology, New University of Lisbon and CEMOP/UNINOVA, 2829-516 Caparica, Portugal
2
Instituto de Tecnologia Química e Biológica Antonio Xavier, Universidade Nova de Lisboa, Avenida da Republica, 2780-157 Oeiras, Portugal
3
Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal
4
Centre for Applied Photonics, Institute for Systems and Computer Engineering Technology and Science (INESC TEC), Rua do Campo Alegre, 687, 4150-179 Porto, Portugal
These authors contributed equally to this work.
Current address: Laboratory of Organic Electronics, Department of Science and Technology, Link
*
Authors to whom correspondence should be addressed.
Received: 1 June 2016 / Revised: 12 September 2016 / Accepted: 26 September 2016 / Published: 7 October 2016
(This article belongs to the Special Issue Advances in Microfluidic Devices for Cell Handling and Analysis)
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Abstract

This paper describes the development of a novel microfluidic platform for multifactorial analysis integrating four label-free detection methods: electrical impedance, refractometry, optical absorption and fluorescence. We present the rationale for the design and the details of the microfabrication of this multifactorial hybrid microfluidic chip. The structure of the platform consists of a three-dimensionally patterned polydimethylsiloxane top part attached to a bottom SU-8 epoxy-based negative photoresist part, where microelectrodes and optical fibers are incorporated to enable impedance and optical analysis. As a proof of concept, the chip functions have been tested and explored, enabling a diversity of applications: (i) impedance-based identification of the size of micro beads, as well as counting and distinguishing of erythrocytes by their volume or membrane properties; (ii) simultaneous determination of the refractive index and optical absorption properties of solutions; and (iii) fluorescence-based bead counting. View Full-Text
Keywords: single cell analysis; label-free methods; impedance spectroscopy; refractometry; optical absorption; hybrid microfluidic chip single cell analysis; label-free methods; impedance spectroscopy; refractometry; optical absorption; hybrid microfluidic chip
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Pereira, F.M.; Bernacka-Wojcik, I.; Ribeiro, R.S.R.; Lobato, M.T.; Fortunato, E.; Martins, R.; Igreja, R.; Jorge, P.A.S.; Águas, H.; Oliva, A.M.G. Hybrid Microfluidic Platform for Multifactorial Analysis Based on Electrical Impedance, Refractometry, Optical Absorption and Fluorescence. Micromachines 2016, 7, 181.

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