Next Article in Journal
In-Situ Testing of the Thermal Diffusivity of Polysilicon Thin Films
Next Article in Special Issue
Baking Powder Actuated Centrifugo-Pneumatic Valving for Automation of Multi-Step Bioassays
Previous Article in Journal
Multiscale and Multimaterial Fabrication: The Challenge Ahead
Article Menu

Export Article

Open AccessArticle
Micromachines 2016, 7(10), 173; doi:10.3390/mi7100173

A Simple Method for Fabrication of Microstructures Using a PDMS Stamp

1
Sensors and MicroActuators Learning Laboratory (SMALL), Department of Electrical Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260, USA
2
Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Nam-Trung Nguyen
Received: 14 June 2016 / Revised: 3 September 2016 / Accepted: 19 September 2016 / Published: 1 October 2016
(This article belongs to the Special Issue Biomedical Microfluidic Devices)
View Full-Text   |   Download PDF [2903 KB, uploaded 1 October 2016]   |  

Abstract

We report a simple method to fabricate PDMS (polydimethylsiloxane) microwell arrays on glass by using a PDMS stamp to study cell-to-cell adhesion. In the cell-to-cell study, a glass substrate is required since glass has better cell attachment. The microwell arrays are replicated from an SU-8 master mold, and then are transferred to a glass substrate by lifting the PDMS stamp, followed by oxygen plasma bonding of the PDMS stamp on the glass substrate. For the cell-to-cell adhesion, four different types of PDMS arrays (e.g., rectangle, bowtie, wide-rhombus, and rhombus) were designed to vary the cell-to-cell contact length. The transfer success rates of the microwell arrays were measured as a function of both the contact area of the PDMS and the glass substrate and the different ratios between the base polymers and the curing agent. This method of generating the microwell arrays will enable a simple and robust construction of PDMS-based devices for various biological applications. View Full-Text
Keywords: PDMS microwell; PDMS microwell transfer; PDMS stamp; cell-to-cell study PDMS microwell; PDMS microwell transfer; PDMS stamp; cell-to-cell study
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Lee, H.; Koh, D.; Xu, L.; Row, S.; Andreadis, S.T.; Oh, K.W. A Simple Method for Fabrication of Microstructures Using a PDMS Stamp. Micromachines 2016, 7, 173.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top