A Simple Method for Fabrication of Microstructures Using a PDMS Stamp
AbstractWe report a simple method to fabricate PDMS (polydimethylsiloxane) microwell arrays on glass by using a PDMS stamp to study cell-to-cell adhesion. In the cell-to-cell study, a glass substrate is required since glass has better cell attachment. The microwell arrays are replicated from an SU-8 master mold, and then are transferred to a glass substrate by lifting the PDMS stamp, followed by oxygen plasma bonding of the PDMS stamp on the glass substrate. For the cell-to-cell adhesion, four different types of PDMS arrays (e.g., rectangle, bowtie, wide-rhombus, and rhombus) were designed to vary the cell-to-cell contact length. The transfer success rates of the microwell arrays were measured as a function of both the contact area of the PDMS and the glass substrate and the different ratios between the base polymers and the curing agent. This method of generating the microwell arrays will enable a simple and robust construction of PDMS-based devices for various biological applications. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Lee, H.; Koh, D.; Xu, L.; Row, S.; Andreadis, S.T.; Oh, K.W. A Simple Method for Fabrication of Microstructures Using a PDMS Stamp. Micromachines 2016, 7, 173.
Lee H, Koh D, Xu L, Row S, Andreadis ST, Oh KW. A Simple Method for Fabrication of Microstructures Using a PDMS Stamp. Micromachines. 2016; 7(10):173.Chicago/Turabian Style
Lee, Hun; Koh, Domin; Xu, Linfeng; Row, Sindhu; Andreadis, Stelios T.; Oh, Kwang W. 2016. "A Simple Method for Fabrication of Microstructures Using a PDMS Stamp." Micromachines 7, no. 10: 173.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.