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Micromachines 2016, 7(10), 175; doi:10.3390/mi7100175

Baking Powder Actuated Centrifugo-Pneumatic Valving for Automation of Multi-Step Bioassays

1
School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland
2
Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland
3
Telecom Physique Strasbourg, Université de Strasbourg, Strasboug CS 10413, France
*
Authors to whom correspondence should be addressed.
Academic Editor: Kwang W. Oh
Received: 4 August 2016 / Revised: 4 September 2016 / Accepted: 19 September 2016 / Published: 1 October 2016
(This article belongs to the Special Issue Biomedical Microfluidic Devices)
View Full-Text   |   Download PDF [4442 KB, uploaded 1 October 2016]   |  

Abstract

We report a new flow control method for centrifugal microfluidic systems; CO2 is released from on-board stored baking powder upon contact with an ancillary liquid. The elevated pressure generated drives the sample into a dead-end pneumatic chamber sealed by a dissolvable film (DF). This liquid incursion wets and dissolves the DF, thus opening the valve. The activation pressure of the DF valve can be tuned by the geometry of the channel upstream of the DF membrane. Through pneumatic coupling with properly dimensioned disc architecture, we established serial cascading of valves, even at a constant spin rate. Similarly, we demonstrate sequential actuation of valves by dividing the disc into a number of distinct pneumatic chambers (separated by DF membranes). Opening these DFs, typically through arrival of a liquid to that location on a disc, permits pressurization of these chambers. This barrier-based scheme provides robust and strictly ordered valve actuation, which is demonstrated by the automation of a multi-step/multi-reagent DNA-based hybridization assay. View Full-Text
Keywords: Lab-on-a-Disc (LoaD); centrifugal microfluidics; dissolvable film valving Lab-on-a-Disc (LoaD); centrifugal microfluidics; dissolvable film valving
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kinahan, D.J.; Renou, M.; Kurzbuch, D.; Kilcawley, N.A.; Bailey, É.; Glynn, M.T.; McDonagh, C.; Ducrée, J. Baking Powder Actuated Centrifugo-Pneumatic Valving for Automation of Multi-Step Bioassays. Micromachines 2016, 7, 175.

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