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Micromachines 2015, 6(5), 634-647; doi:10.3390/mi6050634

Cell Size Discrimination Based on the Measurement of the Equilibrium Velocity in Rectangular Microchannels

1
Fraunhofer ICT-IMM, Carl-Zeiss-Str. 18-20, Mainz 55129, Germany
2
Technische Universität Darmstadt, Schloßgartenstraße 7, Darmstadt 64289, Germany
This paper is an extended version of our paper published in the 2nd International Conference on Microfluidic Handling Systems, Freiburg, Germany, 8–10 October 2014.
*
Author to whom correspondence should be addressed.
Academic Editor: Miko Elwenspoek
Received: 31 December 2014 / Accepted: 19 May 2015 / Published: 22 May 2015
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Abstract

Flow cytometry is a well-established diagnostic tool for cell counting and characterization. It utilizes fluorescence and scattered excitation light simultaneously emitted from cells passing an excitation laser focus to discriminate various cell types and estimate cell size. Here, we apply the principle of spatially modulated emission (SME) to fluorescently stained SUP-B15 cells as a model system for cancer cells and Marinococcus luteus as model for bacteria. We demonstrate that the experimental apparatus is able to detect these model cells and that the results are comparable to those obtained by a commercially available CASY® TT Counter. Furthermore, by examining the velocity distribution of the cells, we observe clear relationships between cell condition/size and cell velocity. Thus, the cell velocity provides information comparable to the scatter signal in conventional flow cytometry. These results indicate that the SME technique is a promising method for simultaneous cell counting and viability characterization. View Full-Text
Keywords: equilibrium velocity; spatially modulated fluorescence emission; cell counter; inertial migration; segré-silberberg effect equilibrium velocity; spatially modulated fluorescence emission; cell counter; inertial migration; segré-silberberg effect
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Schott, L.; Sommer, C.; Wittek, J.; Myagmar, K.; Walther, T.; Baßler, M. Cell Size Discrimination Based on the Measurement of the Equilibrium Velocity in Rectangular Microchannels. Micromachines 2015, 6, 634-647.

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