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Micromachines 2014, 5(4), 1188-1201; doi:10.3390/mi5041188

High Resolution Cell Positioning Based on a Flow Reduction Mechanism for Enhancing Deformability Mapping

1
Department of Mechanical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
2
Department of Micro-Nano Systems Engineering, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya 464-8601, Japan
3
Department of Cardiovascular Medicine, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
Present Address: Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-Ku, Nagoya, Aichi 464-8603, Japan.
*
Author to whom correspondence should be addressed.
Received: 23 September 2014 / Revised: 30 October 2014 / Accepted: 11 November 2014 / Published: 18 November 2014
(This article belongs to the Collection Lab-on-a-Chip)
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Abstract

The dispersion of cell deformability mapping is affected not only by the resolution of the sensing system, but also by cell deformability itself. In order to extract the pure deformability characteristics of cells, it is necessary to improve the resolution of cell actuation in the sensing system, particularly in the case of active sensing, where an actuator is essential. This paper proposes a novel concept, a “flow reduction mechanism”, where a flow is generated by a macroactuator placed outside of a microfluidic chip. The flow can be drastically reduced at the cell manipulation point in a microchannel due to the elasticity embedded into the fluid circuit of the microfluidic system. The great advantage of this approach is that we can easily construct a high resolution cell manipulation system by combining a macro-scale actuator and a macro-scale position sensor, even though the resolution of the actuator is larger than the desired resolution for cell manipulation. Focusing on this characteristic, we successfully achieved the cell positioning based on a visual feedback control with a resolution of 240 nm, corresponding to one pixel of the vision system. We show that the utilization of this positioning system contributes to reducing the dispersion coming from the positioning resolution in the cell deformability mapping. View Full-Text
Keywords: flow reduction mechanism, cell positioning; high resolution; deformability; red blood cell flow reduction mechanism, cell positioning; high resolution; deformability; red blood cell
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Sakuma, S.; Kuroda, K.; Arai, F.; Taniguchi, T.; Ohtani, T.; Sakata, Y.; Kaneko, M. High Resolution Cell Positioning Based on a Flow Reduction Mechanism for Enhancing Deformability Mapping. Micromachines 2014, 5, 1188-1201.

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