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Micromachines 2014, 5(4), 1173-1187; doi:10.3390/mi5041173

Light-Addressable Electrodeposition of Magnetically-Guided Cells Encapsulated in Alginate Hydrogels for Three-Dimensional Cell Patterning

Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung 202-24, Taiwan
Center for Marine Mechatronic Systems (CMMS), National Taiwan Ocean University, Keelung 202-24, Taiwan
Author to whom correspondence should be addressed.
Received: 3 September 2014 / Revised: 5 November 2014 / Accepted: 13 November 2014 / Published: 18 November 2014
(This article belongs to the Special Issue Biomedical Microdevices)
View Full-Text   |   Download PDF [6944 KB, uploaded 18 November 2014]   |  


This paper describes a light-addressable electrolytic system used to perform an electrodeposition of magnetically-guided cells encapsulated in alginate hydrogels using a digital micromirror device (DMD) for three-dimensional cell patterning. In this system, the magnetically-labeled cells were first manipulated into a specific arrangement by changing the orientation of the magnetic field, and then a patterned light illumination was projected onto a photoconductive substrate serving as a photo-anode to cause gelation of calcium alginate through sol-gel transition. By controlling the illumination pattern on the DMD, we first successfully produced cell-encapsulated multilayer alginate hydrogels with different shapes and sizes in each layer via performing multiplexed micropatterning. By combining the magnetically-labeled cells, light-addressable electrodeposition, and orientation of the magnetic fields, we have successfully demonstrated to fabricate two layers of the cell-encapsulated alginate hydrogels, where cells in each layer can be manipulated into cross-directional arrangements that mimic natural tissue. Our proposed method provides a programmable method for the spatiotemporally controllable assembly of cell populations into three-dimensional cell patterning and could have a wide range of biological applications in tissue engineering, toxicology, and drug discovery. View Full-Text
Keywords: electrodeposition; digital micromirror device; calcium alginate electrodeposition; digital micromirror device; calcium alginate

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Huang, S.-H.; Chu, H.-T.; Liou, Y.-M.; Huang, K.-S. Light-Addressable Electrodeposition of Magnetically-Guided Cells Encapsulated in Alginate Hydrogels for Three-Dimensional Cell Patterning. Micromachines 2014, 5, 1173-1187.

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