Abstract: Circulating tumor cells (CTCs) from blood of cancer patients are valuable prognostic markers and enable monitoring responses to therapy. The extremely low number of CTCs makes their isolation and characterization a major technological challenge. For label-free cell identification a novel combination of Raman spectroscopy with a microhole array platform is described that is expected to support high-throughput and multiplex analyses. Raman spectra were registered from regularly arranged cells on the chip with low background noise from the silicon nitride chip membrane. A classification model was trained to distinguish leukocytes from myeloblasts (OCI-AML3) and breast cancer cells (MCF-7 and BT-20). The model was validated by Raman spectra of a mixed cell population. The high spectral quality, low destructivity and high classification accuracy suggests that this approach is promising for Raman activated cell sorting.
Keywords: Raman spectroscopy; circulating tumor cells; cell identification; microhole array chip
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Neugebauer, U.; Kurz, C.; Bocklitz, T.; Berger, T.; Velten, T.; Clement, J.H.; Krafft, C.; Popp, J. Raman-Spectroscopy Based Cell Identification on a Microhole Array Chip. Micromachines 2014, 5, 204-215.
Neugebauer U, Kurz C, Bocklitz T, Berger T, Velten T, Clement JH, Krafft C, Popp J. Raman-Spectroscopy Based Cell Identification on a Microhole Array Chip. Micromachines. 2014; 5(2):204-215.
Neugebauer, Ute; Kurz, Christian; Bocklitz, Thomas; Berger, Tina; Velten, Thomas; Clement, Joachim H.; Krafft, Christoph; Popp, Jürgen. 2014. "Raman-Spectroscopy Based Cell Identification on a Microhole Array Chip." Micromachines 5, no. 2: 204-215.