Micromachines 2014, 5(1), 27-49; doi:10.3390/mi5010027
Review

Ultrasound-Induced Cell–Cell Interaction Studies in a Multi-Well Microplate

1,* email, 1email, 1email, 1email, 1email, 1email and 1,2email
Received: 19 December 2013; in revised form: 23 January 2014 / Accepted: 23 January 2014 / Published: 6 February 2014
(This article belongs to the Special Issue Micro/Nanofluidic Devices for Single Cell Analysis)
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Abstract: This review describes the use of ultrasound for inducing and retaining cell-cell contact in multi-well microplates combined with live-cell fluorescence microscopy. This platform has been used for studying the interaction between natural killer (NK) cells and cancer cells at the level of individual cells. The review includes basic principles of ultrasonic particle manipulation, design criteria when building a multi-well microplate device for this purpose, biocompatibility aspects, and finally, two examples of biological applications: Dynamic imaging of the inhibitory immune synapse, and studies of the heterogeneity in killing dynamics of NK cells interacting with cancer cells.
Keywords: ultrasound; lab-on-a-chip; acoustofluidics; acoustic trapping; natural killer cells
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wiklund, M.; Christakou, A.E.; Ohlin, M.; Iranmanesh, I.; Frisk, T.; Vanherberghen, B.; Önfelt, B. Ultrasound-Induced Cell–Cell Interaction Studies in a Multi-Well Microplate. Micromachines 2014, 5, 27-49.

AMA Style

Wiklund M, Christakou AE, Ohlin M, Iranmanesh I, Frisk T, Vanherberghen B, Önfelt B. Ultrasound-Induced Cell–Cell Interaction Studies in a Multi-Well Microplate. Micromachines. 2014; 5(1):27-49.

Chicago/Turabian Style

Wiklund, Martin; Christakou, Athanasia E.; Ohlin, Mathias; Iranmanesh, Ida; Frisk, Thomas; Vanherberghen, Bruno; Önfelt, Björn. 2014. "Ultrasound-Induced Cell–Cell Interaction Studies in a Multi-Well Microplate." Micromachines 5, no. 1: 27-49.


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