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Toxins 2017, 9(5), 152; doi:10.3390/toxins9050152

Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit

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1
Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
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Risk Assessment Lab for Agro-Products (Beijing), Ministry of Agriculture P.R. China, Beijing 100125, China
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Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, Beijing 100097, China
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School of Science, Shandong University of Technology, Zibo 255000, Shandong, China
*
Author to whom correspondence should be addressed.
Academic Editor: Laura Anfossi
Received: 30 March 2017 / Revised: 21 April 2017 / Accepted: 22 April 2017 / Published: 29 April 2017
(This article belongs to the Section Mycotoxins)
View Full-Text   |   Download PDF [15631 KB, uploaded 29 April 2017]   |  

Abstract

A rapid, portable, and semi-quantitative immunochromatographic strip was developed for rapid and visual detection of alternariol monomethyl ether (AME). For this purpose, the anti-AME monoclonal antibody (mAb) was prepared and identified. AME coupled to bovine serum albumin (BSA) via methyl 4-bromobutanoate was prepared as immunogen. The recoveries of AME in spiked cherry and orange fruits determined by competitive ELISA were 86.1% and 80.7%, respectively. A colloidal gold nanoparticle (CGN) and CGNs-mAb conjugate were synthesized, and on this basis, a competitive colloidal gold immunochromatographic strip was developed and applied to the detection of AME toxin in fruit samples. The intensity of red density of the test line (T line) is inversely proportional to AME concentration in the range 0.1–10 ng/mL. The visual limit of detection (LOD) of AME was found to be about 10 ng/mL. The semi-quantitative detection can be completed in 10 min. Moreover, the immunochromatographic strip has lower cross-reactivity with AME analogues, and it has a good stability performance (following 3 months of storage). Hence, the colloidal gold immunochromatographic strip could be used as a semi-quantitative tool for the on-site, rapid, and visual detection of AME in fruit. View Full-Text
Keywords: alternariol monomethyl ether (AME); monoclonal antibody; immunochromatographic strip test; colloidal gold nanoparticle; competitive ELISA alternariol monomethyl ether (AME); monoclonal antibody; immunochromatographic strip test; colloidal gold nanoparticle; competitive ELISA
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Man, Y.; Liang, G.; Jia, F.; Li, A.; Fu, H.; Wang, M.; Pan, L. Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit. Toxins 2017, 9, 152.

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