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Toxins 2017, 9(3), 74; doi:10.3390/toxins9030074

Antivenom Evaluation by Electrophysiological Analysis

1
Instituto de Biotecnologia, National Autonomous University of México, Av. Univeresidad 2001, Col. Chamilpa, Cuernavaca 62210, Morelos, Mexico
2
Instituto Bioclón S.A. de C.V., Calle Miguel Laurent 427, Del. Benito Juarez, Ciudad de México 03100, Mexico
3
Laboratorios Silanes, S.A. de C.V., Amores 1304, Colonia del Valle, Ciudad de México 03100, Mexico
*
Authors to whom correspondence should be addressed.
Academic Editor: Eivind Undheim
Received: 31 January 2017 / Revised: 21 February 2017 / Accepted: 21 February 2017 / Published: 23 February 2017
(This article belongs to the Section Animal Venoms)
View Full-Text   |   Download PDF [2578 KB, uploaded 23 February 2017]   |  

Abstract

Scorpion stings on humans are medically relevant because they may contain toxins that specifically target ion channels. During antivenom production, pharmaceutical companies must use a large number of experimental animals to ensure the antivenom’s efficacy according to pharmacopeia methods. Here we present an electrophysiological alternative for the evaluation of horse antivenoms produced against two species of Moroccan scorpions: Buthus mardochei and Androctonus mauretanicus. Human sodium and potassium channels and acetylcholine nicotinic receptors were analyzed by standard patch-clamp techniques. The results showed that the antivenom is capable of reversing ion current disruption caused by the venom application. We propose the use of this in vitro technique for antivenom evaluation as an alternative to using a large number of live animals. View Full-Text
Keywords: antivenom; horse immunoglobulin; human ion channels scorpion venoms; sodium channel; patch-clamp; electrophysiology antivenom; horse immunoglobulin; human ion channels scorpion venoms; sodium channel; patch-clamp; electrophysiology
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Restano-Cassulini, R.; Garcia, W.; Paniagua-Solís, J.F.; Possani, L.D. Antivenom Evaluation by Electrophysiological Analysis. Toxins 2017, 9, 74.

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