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Toxins 2017, 9(2), 70; doi:10.3390/toxins9020070

Determination of Mycotoxin Production of Fusarium Species in Genetically Modified Maize Varieties by Quantitative Flow Immunocytometry

1
Agro-Environmental Research Institute, National Research and Innovation Centre, Herman Ottó út 15, H-1022 Budapest, Hungary
2
Soft Flow Hungary R&D Ltd., Ürögi fasor 2/A, H-7634 Pécs, Hungary
*
Author to whom correspondence should be addressed.
Academic Editor: Ibtisam E. Tothill
Received: 28 December 2016 / Revised: 25 January 2017 / Accepted: 8 February 2017 / Published: 22 February 2017
(This article belongs to the Section Mycotoxins)
View Full-Text   |   Download PDF [2293 KB, uploaded 22 February 2017]   |  

Abstract

Levels of mycotoxins produced by Fusarium species in genetically modified (GM) and near-isogenic maize, were determined using multi-analyte, microbead-based flow immunocytometry with fluorescence detection, for the parallel quantitative determination of fumonisin B1, deoxynivalenol, zearalenone, T-2, ochratoxin A, and aflatoxin B1. Maize varieties included the genetic events MON 810 and DAS-59122-7, and their isogenic counterparts. Cobs were artificially infested by F. verticillioides and F. proliferatum conidia, and contained F. graminearum and F. sporotrichoides natural infestation. The production of fumonisin B1 and deoxynivalenol was substantially affected in GM maize lines: F. verticillioides, with the addition of F. graminearum and F. sporotrichoides, produced significantly lower levels of fumonisin B1 (~300 mg·kg−1) in DAS-59122-7 than in its isogenic line (~580 mg·kg−1), while F. proliferatum, in addition to F. graminearum and F. sporotrichoides, produced significantly higher levels of deoxynivalenol (~18 mg·kg−1) in MON 810 than in its isogenic line (~5 mg·kg−1). Fusarium verticillioides, with F. graminearum and F. sporotrichoides, produced lower amounts of deoxynivalenol and zearalenone than F. proliferatum, with F. graminearum and F. sporotrichoides. T-2 toxin production remained unchanged when considering the maize variety. The results demonstrate the utility of the Fungi-Plex™ quantitative flow immunocytometry method, applied for the high throughput parallel determination of the target mycotoxins. View Full-Text
Keywords: flow cytometry; immunoanalysis; maize; MON 810; DAS-59122-7; fumonisin B1; deoxynivalenol; zearalenone; T-2; ochratoxin A; aflatoxin B1 flow cytometry; immunoanalysis; maize; MON 810; DAS-59122-7; fumonisin B1; deoxynivalenol; zearalenone; T-2; ochratoxin A; aflatoxin B1
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MDPI and ACS Style

Bánáti, H.; Darvas, B.; Fehér-Tóth, S.; Czéh, Á.; Székács, A. Determination of Mycotoxin Production of Fusarium Species in Genetically Modified Maize Varieties by Quantitative Flow Immunocytometry. Toxins 2017, 9, 70.

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