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Toxins 2017, 9(10), 328; https://doi.org/10.3390/toxins9100328

A Monoclonal–Monoclonal Antibody Based Capture ELISA for Abrin

1
Foodborne Toxin Detection and Prevention Research Unit, Western Regional Research Center, Agricultural Research Services, United States Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA
2
United States Department of Homeland Security, Washington, DC 20528, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Julien Barbier and Daniel Gillet
Received: 1 September 2017 / Revised: 9 October 2017 / Accepted: 13 October 2017 / Published: 18 October 2017
(This article belongs to the Special Issue Ribosome Inactivating Toxins)
View Full-Text   |   Download PDF [2185 KB, uploaded 18 October 2017]   |  

Abstract

Abrin, one of the most highly potent toxins in the world, is derived from the plant, Abrus precatorius. Because of its high toxicity, it poses potential bioterror risks. Therefore, a need exists for new reagents and technologies that would be able to rapidly detect abrin contamination as well as lead to new therapeutics. We report here a group of abrin-specific monoclonal antibodies (mAbs) that recognize abrin A-chain, intact A–B chain toxin, and agglutinin by Western blot. Additionally, these mAbs were evaluated for their ability to serve as capture antibodies for a sandwich (capture) ELISA. All possible capture–detector pairs were evaluated and the best antibody pair identified and optimized for a capture ELISA. The capture ELISA based on this capture–detector mAb pair had a limit of detection (L.O.D) of ≈1 ng/mL measured using three independent experiments. The assay did not reveal any false positives with extracts containing other potential ribosome-inactivating proteins (RIPs). Thus, this new capture ELISA uses mAbs for both capture and detection; has no cross-reactivity against other plant RIPs; and has a sensitivity comparable to other reported capture ELISAs using polyclonal antibodies as either capture or detector. View Full-Text
Keywords: abrin; abrus precatorius; ELISA; monoclonal antibodies; ribosome-inactivating protein abrin; abrus precatorius; ELISA; monoclonal antibodies; ribosome-inactivating protein
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Tam, C.C.; Cheng, L.W.; He, X.; Merrill, P.; Hodge, D.; Stanker, L.H. A Monoclonal–Monoclonal Antibody Based Capture ELISA for Abrin. Toxins 2017, 9, 328.

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