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Toxins 2016, 8(6), 184; doi:10.3390/toxins8060184

Anti-Human Endoglin (hCD105) Immunotoxin—Containing Recombinant Single Chain Ribosome-Inactivating Protein Musarmin 1

1
Department of Biochemistry and Molecular Biology, University of Valladolid, 47005 Valladolid, Spain
2
Bioforge, University of Valladolid, Spain and Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 47002 Valladolid, Spain
3
Department of Nutrition and Bromatology, University of Valladolid, 47005 Valladolid, Spain
4
Department of Pharmacy & Pharmaceutical Technology and University Institute of Industrial Pharmacy (IUFI), Complutense University of Madrid, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Pang-Chui Shaw
Received: 14 April 2016 / Revised: 24 May 2016 / Accepted: 3 June 2016 / Published: 10 June 2016
(This article belongs to the Collection Immunotoxins 2016)
View Full-Text   |   Download PDF [1393 KB, uploaded 10 June 2016]   |  

Abstract

Endoglin (CD105) is an accessory component of the TGF-β receptor complex, which is expressed in a number of tissues and over-expressed in the endothelial cells of tumor neovasculature. Targeting endoglin with immunotoxins containing type 2 ribosome-inactivating proteins has proved an effective tool to reduce blood supply to B16 mice tumor xenografts. We prepared anti-endoglin immunotoxin (IT)—containing recombinant musarmin 1 (single chain ribosome-inactivating proteins) linked to the mouse anti-human CD105 44G4 mouse monoclonal antibody via N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). The immunotoxin specifically killed L929 fibroblast mouse cells transfected with the short form of human endoglin with IC50 values in the range of 5 × 10−10 to 10−9 M. View Full-Text
Keywords: recombinant musarmin; endoglin; CD105; 44G4; anti-endoglin monoclonal antibody; anti-tumor therapy; cancer therapy recombinant musarmin; endoglin; CD105; 44G4; anti-endoglin monoclonal antibody; anti-tumor therapy; cancer therapy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Barriuso, B.; Antolín, P.; Arias, F.J.; Girotti, A.; Jiménez, P.; Cordoba-Diaz, M.; Cordoba-Diaz, D.; Girbés, T. Anti-Human Endoglin (hCD105) Immunotoxin—Containing Recombinant Single Chain Ribosome-Inactivating Protein Musarmin 1. Toxins 2016, 8, 184.

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