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Toxins 2016, 8(12), 375; doi:10.3390/toxins8120375

QuEChERS Purification Combined with Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry for Simultaneous Quantification of 25 Mycotoxins in Cereals

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1,2
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1,2
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1,2
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1,2
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1,2,*
1
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
Laboratory of Quality and Safety Risk Assessment for Cereal Products (Beijing), Ministry of Agriculture, Beijing 100081, China
*
Author to whom correspondence should be addressed.
Academic Editor: Aldo Laganà
Received: 4 May 2016 / Revised: 8 December 2016 / Accepted: 9 December 2016 / Published: 15 December 2016
(This article belongs to the Special Issue LC-MS/MS Method for Mycotoxin Analysis)
View Full-Text   |   Download PDF [1706 KB, uploaded 15 December 2016]   |  

Abstract

A method based on the QuEChERS (quick, easy, cheap, effective, rugged, and safe) purification combined with ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS), was optimized for the simultaneous quantification of 25 mycotoxins in cereals. Samples were extracted with a solution containing 80% acetonitrile and 0.1% formic acid, and purified with QuEChERS before being separated by a C18 column. The mass spectrometry was conducted by using positive electrospray ionization (ESI+) and multiple reaction monitoring (MRM) models. The method gave good linear relations with regression coefficients ranging from 0.9950 to 0.9999. The detection limits ranged from 0.03 to 15.0 µg·kg−1, and the average recovery at three different concentrations ranged from 60.2% to 115.8%, with relative standard deviations (RSD%) varying from 0.7% to 19.6% for the 25 mycotoxins. The method is simple, rapid, accurate, and an improvement compared with the existing methods published so far. View Full-Text
Keywords: ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS); QuEChERS; mycotoxin; cereals ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS); QuEChERS; mycotoxin; cereals
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Sun, J.; Li, W.; Zhang, Y.; Hu, X.; Wu, L.; Wang, B. QuEChERS Purification Combined with Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry for Simultaneous Quantification of 25 Mycotoxins in Cereals. Toxins 2016, 8, 375.

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