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Toxins 2015, 7(9), 3771-3784; doi:10.3390/toxins7093771

Titanium Dioxide Nanoparticles (TiO2) Quenching Based Aptasensing Platform: Application to Ochratoxin A Detection

1
BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France
2
Biosensor Lab, Department of Chemistry, BITS, Pilani- K. K. Birla Goa Campus, Zuarinagar, Goa 403726, India
3
Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology (CIIT), Lahore 54000, Pakistan
4
Department of Biosciences and Biotechnology, Banasthali University, Rajasthan 304022, India
*
Author to whom correspondence should be addressed.
Academic Editor: Michelangelo Pascale
Received: 30 August 2015 / Revised: 15 September 2015 / Accepted: 16 September 2015 / Published: 22 September 2015
(This article belongs to the Collection Biorecognition Assays for Mycotoxins)
View Full-Text   |   Download PDF [1637 KB, uploaded 22 September 2015]   |  

Abstract

We demonstrate for the first time, the development of titanium dioxide nanoparticles (TiO2) quenching based aptasensing platform for detection of target molecules. TiO2 quench the fluorescence of FAM-labeled aptamer (fluorescein labeled aptamer) upon the non-covalent adsorption of fluorescent labeled aptamer on TiO2 surface. When OTA interacts with the aptamer, it induced aptamer G-quadruplex complex formation, weakens the interaction between FAM-labeled aptamer and TiO2, resulting in fluorescence recovery. As a proof of concept, an assay was employed for detection of Ochratoxin A (OTA). At optimized experimental condition, the obtained limit of detection (LOD) was 1.5 nM with a good linearity in the range 1.5 nM to 1.0 µM for OTA. The obtained results showed the high selectivity of assay towards OTA without interference to structurally similar analogue Ochratoxin B (OTB). The developed aptamer assay was evaluated for detection of OTA in beer sample and recoveries were recorded in the range from 94.30%–99.20%. Analytical figures of the merits of the developed aptasensing platform confirmed its applicability to real samples analysis. However, this is a generic aptasensing platform and can be extended for detection of other toxins or target analyte. View Full-Text
Keywords: titanium dioxide nanoparticles; fluorescently labeled aptamer; aptamer assay; ochratoxin A; beer; quenching titanium dioxide nanoparticles; fluorescently labeled aptamer; aptamer assay; ochratoxin A; beer; quenching
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Sharma, A.; Hayat, A.; Mishra, R.K.; Catanante, G.; Bhand, S.; Marty, J.L. Titanium Dioxide Nanoparticles (TiO2) Quenching Based Aptasensing Platform: Application to Ochratoxin A Detection. Toxins 2015, 7, 3771-3784.

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