Next Article in Journal
Conotoxin Interactions with α9α10-nAChRs: Is the α9α10-Nicotinic Acetylcholine Receptor an Important Therapeutic Target for Pain Management?
Next Article in Special Issue
A Magnetic Nanoparticle Based Enzyme-Linked Immunosorbent Assay for Sensitive Quantification of Zearalenone in Cereal and Feed Samples
Previous Article in Journal
Suppression of Aflatoxin Biosynthesis in Aspergillus flavus by 2-Phenylethanol Is Associated with Stimulated Growth and Decreased Degradation of Branched-Chain Amino Acids
Previous Article in Special Issue
Titanium Dioxide Nanoparticles (TiO2) Quenching Based Aptasensing Platform: Application to Ochratoxin A Detection
Article Menu

Export Article

Open AccessArticle
Toxins 2015, 7(10), 3903-3915; doi:10.3390/toxins7103903

Development and Evaluation of Monoclonal Antibodies for Paxilline

Bacterial Foodborne Pathogens and Mycology Research Unit, USDA-ARS-NCAUR, 1815 North University Street, Peoria, IL 61604, USA
Academic Editor: Laura Anfossi
Received: 14 August 2015 / Revised: 8 September 2015 / Accepted: 22 September 2015 / Published: 25 September 2015
(This article belongs to the Collection Biorecognition Assays for Mycotoxins)
View Full-Text   |   Download PDF [994 KB, uploaded 25 September 2015]   |  

Abstract

Paxilline (PAX) is a tremorgenic mycotoxin that has been found in perennial ryegrass infected with Acremonium lolii. To facilitate screening for this toxin, four murine monoclonal antibodies (mAbs) were developed. In competitive indirect enzyme-linked immunosorbent assays (CI-ELISAs) the concentrations of PAX required to inhibit signal development by 50% (IC50s) ranged from 1.2 to 2.5 ng/mL. One mAb (2-9) was applied to the detection of PAX in maize silage. The assay was sensitive to the effects of solvents, with 5% acetonitrile or 20% methanol causing a two-fold or greater increase in IC50. For analysis of silage samples, extracts were cleaned up by adsorbing potential matrix interferences onto a solid phase extraction column. The non-retained extract was then diluted with buffer to reduce solvent content prior to assay. Using this method, the limit of detection for PAX in dried silage was 15 µg/kg and the limit of quantification was 90 µg/kg. Recovery from samples spiked over the range of 100 to 1000 µg/kg averaged 106% ± 18%. The assay was applied to 86 maize silage samples, with many having detectable, but none having quantifiable, levels of PAX. The results suggest the CI-ELISA can be applied as a sensitive technique for the screening of PAX in maize silage. View Full-Text
Keywords: paxilline; tremorgen; antibody; immunoassay; mycotoxin; silage paxilline; tremorgen; antibody; immunoassay; mycotoxin; silage
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Maragos, C.M. Development and Evaluation of Monoclonal Antibodies for Paxilline. Toxins 2015, 7, 3903-3915.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top