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Toxins 2015, 7(9), 3700-3714; doi:10.3390/toxins7093700

Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B2 and B3 in Corn by High-Resolution LC-Orbitrap MS

1
National Agriculture and Food Research Organization (NARO), National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan
2
Thermo Fisher Scientific, C-2F, 3-9 Moriya-cho, Yokohama-shi, Kanagawa 221-0022, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Sven Dänicke
Received: 30 June 2015 / Revised: 3 September 2015 / Accepted: 7 September 2015 / Published: 16 September 2015
(This article belongs to the Collection Fusarium Toxins – Relevance for Human and Animal Health)
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Abstract

The existence of glucose conjugates of fumonisin B2 (FB2) and fumonisin B3 (FB3) in corn powder was confirmed for the first time. These “bound-fumonisins” (FB2 and FB3 bound to glucose) were identified as N-(1-deoxy-d-fructos-1-yl) fumonisin B2 (NDfrc-FB2) and N-(1-deoxy-d-fructos-1-yl) fumonisin B3 (NDfrc-FB3) respectively, based on the accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) analysis. Treatment on NDfrc-FB2 and NDfrc-FB3 with the o-phthalaldehyde (OPA) reagent also supported that d-glucose binding to FB2 and FB3 molecules occurred to their primary amine residues. View Full-Text
Keywords: LC-Orbitrap MS; fumonisin; Fusarium; corn; N-(1-deoxy-d-fructos-1-yl) fumonisin LC-Orbitrap MS; fumonisin; Fusarium; corn; N-(1-deoxy-d-fructos-1-yl) fumonisin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Matsuo, Y.; Takahara, K.; Sago, Y.; Kushiro, M.; Nagashima, H.; Nakagawa, H. Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B2 and B3 in Corn by High-Resolution LC-Orbitrap MS. Toxins 2015, 7, 3700-3714.

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