Next Article in Journal
Immune Checkpoint Blockade in Cancer Treatment: A Double-Edged Sword Cross-Targeting the Host as an “Innocent Bystander”
Next Article in Special Issue
Comments on Environmental and Sanitary Aspects of the Scorpionism by Tityus trivittatus in Buenos Aires City, Argentina
Previous Article in Journal
Monoclonal Antibody Therapy and Renal Transplantation: Focus on Adverse Effects
Previous Article in Special Issue
Characterization of a Novel BmαTX47 Toxin Modulating Sodium Channels: The Crucial Role of Expression Vectors in Toxin Pharmacological Activity
Toxins 2014, 6(3), 892-913; doi:10.3390/toxins6030892
Article

Electrophysiological Characterization of Ts6 and Ts7, K+ Channel Toxins Isolated through an Improved Tityus serrulatus Venom Purification Procedure

1,†
, 1,†
, 2
, 1
, 1
, 2
 and 1,*
Received: 12 December 2013; in revised form: 24 January 2014 / Accepted: 17 February 2014 / Published: 28 February 2014
(This article belongs to the Special Issue Scorpion Toxins)
View Full-Text   |   Download PDF [1306 KB, uploaded 28 February 2014]   |   Browse Figures
Abstract: In Brazil, Tityus serrulatus (Ts) is the species responsible for most of the scorpion related accidents. Among the Ts toxins, the neurotoxins with action on potassium channels (α-KTx) present high interest, due to their effect in the envenoming process and the ion channel specificity they display. The α-KTx toxins family is the most relevant because its toxins can be used as therapeutic tools for specific target cells. The improved isolation method provided toxins with high resolution, obtaining pure Ts6 and Ts7 in two chromatographic steps. The effects of Ts6 and Ts7 toxins were evaluated in 14 different types of potassium channels using the voltage-clamp technique with two-microelectrodes. Ts6 toxin shows high affinity for Kv1.2, Kv1.3 and Shaker IR, blocking these channels in low concentrations. Moreover, Ts6 blocks the Kv1.3 channel in picomolar concentrations with an IC50 of 0.55 nM and therefore could be of valuable assistance to further designing immunosuppressive therapeutics. Ts7 toxin blocks multiple subtypes channels, showing low selectivity among the channels analyzed. This work also stands out in its attempt to elucidate the residues important for interacting with each channel and, in the near future, to model a desired drug.
Keywords: Tityus serrulatus; neurotoxins; potassium channels; α-KTx; scorpion venom Tityus serrulatus; neurotoxins; potassium channels; α-KTx; scorpion venom
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Cerni, F.A.; Pucca, M.B.; Peigneur, S.; Cremonez, C.M.; Bordon, K.C.F.; Tytgat, J.; Arantes, E.C. Electrophysiological Characterization of Ts6 and Ts7, K+ Channel Toxins Isolated through an Improved Tityus serrulatus Venom Purification Procedure. Toxins 2014, 6, 892-913.

AMA Style

Cerni FA, Pucca MB, Peigneur S, Cremonez CM, Bordon KCF, Tytgat J, Arantes EC. Electrophysiological Characterization of Ts6 and Ts7, K+ Channel Toxins Isolated through an Improved Tityus serrulatus Venom Purification Procedure. Toxins. 2014; 6(3):892-913.

Chicago/Turabian Style

Cerni, Felipe A.; Pucca, Manuela B.; Peigneur, Steve; Cremonez, Caroline M.; Bordon, Karla C.F.; Tytgat, Jan; Arantes, Eliane C. 2014. "Electrophysiological Characterization of Ts6 and Ts7, K+ Channel Toxins Isolated through an Improved Tityus serrulatus Venom Purification Procedure." Toxins 6, no. 3: 892-913.


Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert