Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Toxins 2014, 6(3), 816-829; doi:10.3390/toxins6030816
Article

Characterization of a Novel BmαTX47 Toxin Modulating Sodium Channels: The Crucial Role of Expression Vectors in Toxin Pharmacological Activity

1
, 1
, 1
, 1
, 2
, 1,*  and 1,*
Received: 4 December 2013; in revised form: 30 December 2013 / Accepted: 20 January 2014 / Published: 26 February 2014
(This article belongs to the Special Issue Scorpion Toxins)
View Full-Text   |   Download PDF [1589 KB, uploaded 26 February 2014]
Abstract: Long-chain scorpion toxins with four disulfide bridges exhibit various pharmacological features towards the different voltage-gated sodium channel subtypes. However, the toxin production still remains a huge challenge. Here, we reported the effects of different expression vectors on the pharmacological properties of a novel toxin BmαTX47 from the scorpion Buthus martensii Karsch. The recombinant BmαTX47 was obtained using the expression vector pET-14b and pET-28a, respectively. Pharmacological experiments showed that the recombinant BmαTX47 was a new α-scorpion toxin which could inhibit the fast inactivation of rNav1.2, mNav1.4 and hNav1.5 channels. Importantly, the different expression vectors were found to strongly affect BmαTX47 pharmacological activities while toxins were obtained by the same expression and purification procedures. When 10 µM recombinant BmαTX47 from the pET-28a vector was applied, the values of I5ms/Ipeak for rNav1.2, mNav1.4 and hNav1.5 channels were 44.12% ± 3.17%, 25.40% ± 4.89% and 65.34% ± 3.86%, respectively, which were better than those values of 11.33% ± 1.46%, 15.96% ± 1.87% and 5.24% ± 2.38% for rNav1.2, mNav1.4 and hNav1.5 channels delayed by 10 µM recombinant BmαTX47 from the pET-14b vector. The dose-response experiments further indicated the EC50 values of recombinant BmαTX47 from the pET-28a vector were 7262.9 ± 755.9 nM for rNav1.2 channel and 1005.8 ± 118.6 nM for hNav1.5 channel, respectively. Together, these findings highlighted the important role of expression vectors in scorpion toxin pharmacological properties, which would accelerate the understanding of the structure-function relationships of scorpion toxins and promote the potential application of toxins in the near future.
Keywords: Buthus martensii Karsch; BmαTX47; recombinant expression; sodium channels; pET-28a vector; pET-14b vector Buthus martensii Karsch; BmαTX47; recombinant expression; sodium channels; pET-28a vector; pET-14b vector
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Li, T.; Xu, L.; Liu, H.; He, Y.; Liang, S.; Li, W.; Wu, Y. Characterization of a Novel BmαTX47 Toxin Modulating Sodium Channels: The Crucial Role of Expression Vectors in Toxin Pharmacological Activity. Toxins 2014, 6, 816-829.

AMA Style

Li T, Xu L, Liu H, He Y, Liang S, Li W, Wu Y. Characterization of a Novel BmαTX47 Toxin Modulating Sodium Channels: The Crucial Role of Expression Vectors in Toxin Pharmacological Activity. Toxins. 2014; 6(3):816-829.

Chicago/Turabian Style

Li, Tian; Xu, Lingna; Liu, Honglian; He, Yawen; Liang, Songping; Li, Wenxin; Wu, Yingliang. 2014. "Characterization of a Novel BmαTX47 Toxin Modulating Sodium Channels: The Crucial Role of Expression Vectors in Toxin Pharmacological Activity." Toxins 6, no. 3: 816-829.



Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert