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Toxins 2013, 5(8), 1447-1461; doi:10.3390/toxins5081447
Article

Non-Linear Relationships between Aflatoxin B1 Levels and the Biological Response of Monkey Kidney Vero Cells

1,* , 1
, 1
 and 2
Received: 24 July 2013; in revised form: 31 July 2013 / Accepted: 2 August 2013 / Published: 14 August 2013
(This article belongs to the Special Issue Advances in Toxin Detection)
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Abstract: Aflatoxin-producing fungi contaminate food and feed during pre-harvest, storage and processing periods. Once consumed, aflatoxins (AFs) accumulate in tissues, causing illnesses in animals and humans. Most human exposure to AF seems to be a result of consumption of contaminated plant and animal products. The policy of blending and dilution of grain containing higher levels of aflatoxins with uncontaminated grains for use in animal feed implicitly assumes that the deleterious effects of low levels of the toxins are linearly correlated to concentration. This assumption may not be justified, since it involves extrapolation of these nontoxic levels in feed, which are not of further concern. To develop a better understanding of the significance of low dose effects, in the present study, we developed quantitative methods for the detection of biologically active aflatoxin B1 (AFB1) in Vero cells by two independent assays: the green fluorescent protein (GFP) assay, as a measure of protein synthesis by the cells, and the microculture tetrazolium (MTT) assay, as a measure of cell viability. The results demonstrate a non-linear dose-response relationship at the cellular level. AFB1 at low concentrations has an opposite biological effect to higher doses that inhibit protein synthesis. Additional studies showed that heat does not affect the stability of AFB1 in milk and that the Vero cell model can be used to determine the presence of bioactive AFB1 in spiked beef, lamb and turkey meat. The implication of the results for the cumulative effects of low amounts of AFB1 in numerous foods is discussed.
Keywords: aflatoxin B1; Vero cells; bioactivity; toxicity; non-linear dose effect; milk; meat; MTT assay; GFP assay; food safety aflatoxin B1; Vero cells; bioactivity; toxicity; non-linear dose effect; milk; meat; MTT assay; GFP assay; food safety
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Rasooly, R.; Hernlem, B.; He, X.; Friedman, M. Non-Linear Relationships between Aflatoxin B1 Levels and the Biological Response of Monkey Kidney Vero Cells. Toxins 2013, 5, 1447-1461.

AMA Style

Rasooly R, Hernlem B, He X, Friedman M. Non-Linear Relationships between Aflatoxin B1 Levels and the Biological Response of Monkey Kidney Vero Cells. Toxins. 2013; 5(8):1447-1461.

Chicago/Turabian Style

Rasooly, Reuven; Hernlem, Bradley; He, Xiaohua; Friedman, Mendel. 2013. "Non-Linear Relationships between Aflatoxin B1 Levels and the Biological Response of Monkey Kidney Vero Cells." Toxins 5, no. 8: 1447-1461.


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