Toxins 2013, 5(6), 1167-1179; doi:10.3390/toxins5061167
Article

Determinants of pH-Dependent Modulation of Translocation in Dermonecrotic G-Protein-Deamidating Toxins

Received: 17 May 2013; in revised form: 28 May 2013 / Accepted: 4 June 2013 / Published: 14 June 2013
(This article belongs to the Special Issue Novel Properties of Well-Characterized Toxins)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Cytotoxic necrotizing factors from E. coli (CNF1, CNF2) and Yersinia (CNFy) share N-terminal sequence similarity with Pasteurella multocida toxin (PMT). This common N-terminal region harbors the receptor-binding and translocation domains that mediate uptake and delivery of the C-terminal catalytic cargo domains into the host cytosol. Subtle variations in the N-terminal ~500 amino acids of CNFs and PMT could allow for selective recognition of cellular receptors and thus, selective target cell specificity. Through studies with cellular inhibitors, we have identified an additional novel function for this region in modulating responses of these toxin proteins to changes in pH during intoxication and delivery of the catalytic cargo domain into the cytosol.
Keywords: cytotoxic necrotizing factor; Pasteurella multocida toxin; dermonecrotic toxin; endosomal acidification; intoxication; drug-delivery; toxin-based therapeutics
PDF Full-text Download PDF Full-Text [647 KB, Updated Version, uploaded 19 June 2013 16:42 CEST]
The original version is still available [436 KB, uploaded 14 June 2013 12:08 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Repella, T.L.; Ho, M.; Wilson, B.A. Determinants of pH-Dependent Modulation of Translocation in Dermonecrotic G-Protein-Deamidating Toxins. Toxins 2013, 5, 1167-1179.

AMA Style

Repella TL, Ho M, Wilson BA. Determinants of pH-Dependent Modulation of Translocation in Dermonecrotic G-Protein-Deamidating Toxins. Toxins. 2013; 5(6):1167-1179.

Chicago/Turabian Style

Repella, Tana L.; Ho, Mengfei; Wilson, Brenda A. 2013. "Determinants of pH-Dependent Modulation of Translocation in Dermonecrotic G-Protein-Deamidating Toxins." Toxins 5, no. 6: 1167-1179.

Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert