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Toxins 2012, 4(6), 430-454; doi:10.3390/toxins4060430
Review

Monoclonal Antibodies and Toxins—A Perspective on Function and Isotype

1
 and
1,2,*
Received: 29 April 2012 / Revised: 6 June 2012 / Accepted: 7 June 2012 / Published: 11 June 2012
(This article belongs to the Special Issue Toxin-Antibody Interactions)
View Full-Text   |   Download PDF [526 KB, 12 June 2012; original version 11 June 2012]   |   Browse Figures

Abstract

Antibody therapy remains the only effective treatment for toxin-mediated diseases. The development of hybridoma technology has allowed the isolation of monoclonal antibodies (mAbs) with high specificity and defined properties, and numerous mAbs have been purified and characterized for their protective efficacy against different toxins. This review summarizes the mAb studies for 6 toxins—Shiga toxin, pertussis toxin, anthrax toxin, ricin toxin, botulinum toxin, and Staphylococcal enterotoxin B (SEB)—and analyzes the prevalence of mAb functions and their isotypes. Here we show that most toxin-binding mAbs resulted from immunization are non-protective and that mAbs with potential therapeutic use are preferably characterized. Various common practices and caveats of protection studies are discussed, with the goal of providing insights for the design of future research on antibody-toxin interactions.
Keywords: antibody; neutralization; protection; clearance; vaccine; therapeutics; isotype; animal model; in vivo; disease enhancement antibody; neutralization; protection; clearance; vaccine; therapeutics; isotype; animal model; in vivo; disease enhancement
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chow, S.-K.; Casadevall, A. Monoclonal Antibodies and Toxins—A Perspective on Function and Isotype. Toxins 2012, 4, 430-454.

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