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Toxins 2012, 4(6), 455-475; doi:10.3390/toxins4060455

Multimodal Protein Constructs for Herbivore Insect Control

Département de phytologie, Centre de recherche en horticulture, Pavillon des Services, Université Laval, Québec QC, G1V 0A6, Canada
* Author to whom correspondence should be addressed.
Received: 8 March 2012 / Revised: 1 June 2012 / Accepted: 5 June 2012 / Published: 12 June 2012
(This article belongs to the Special Issue Insecticidal Toxins)
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Transgenic plants expressing combinations of microbial or plant pesticidal proteins represent a promising tool for the efficient, durable control of herbivorous insects. In this review we describe current strategies devised for the heterologous co-expression of pesticidal proteins in planta, some of which have already shown usefulness in plant protection. Emphasis is placed on protein engineering strategies involving the insertion of single DNA constructs within the host plant genome. Multimodal fusion proteins integrating complementary pesticidal functions along a unique polypeptide are first considered, taking into account the structural constraints associated with protein or protein domain grafting to biologically active proteins. Strategies that allow for the co- or post-translational release of two or more pesticidal proteins are then considered, including polyprotein precursors releasing free proteins upon proteolytic cleavage, and multicistronic transcripts for the parallel translation of single protein-encoding mRNA sequences.
Keywords: insect-resistant transgenic plants; Bt plants; Cry toxins; defense proteins; gene stacking; polyproteins; protein pyramiding; fusion proteins insect-resistant transgenic plants; Bt plants; Cry toxins; defense proteins; gene stacking; polyproteins; protein pyramiding; fusion proteins
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sainsbury, F.; Benchabane, M.; Goulet, M.-C.; Michaud, D. Multimodal Protein Constructs for Herbivore Insect Control. Toxins 2012, 4, 455-475.

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