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• Kong, Q
• Zhai, C
• Guan, B
• Li, C
• Shan, S
• Yu, J
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• Kong, Q
• Zhai, C
• Guan, B
• Li, C
• Shan, S
• Yu, J
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• Zhai, C
• Guan, B
• Li, C
• Shan, S
• Yu, J

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Toxins 2012, 4(11), 1181-1195; doi:10.3390/toxins4111181

Article

Mathematic Modeling for Optimum Conditions on Aflatoxin B₁ Degradation by the Aerobic Bacterium Rhodococcus erythropolis

Qing Kong ^1,* , Cuiping Zhai ¹ , Bin Guan ¹ , Chunjuan Li ² , Shihua Shan ²  and Jiujiang Yu ³

¹ School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China ² Shandong Peanut Research Institute, Qingdao, Shandong 266100, China ³ Department of Agriculture (USDA), Agricultural Research Service (ARS), Southern Regional Research Center, New Orleans, LA 70124, USA

* Author to whom correspondence should be addressed.

Received: 25 July 2012; in revised form: 8 October 2012 / Accepted: 31 October 2012 / Published: 6 November 2012

(This article belongs to the Special Issue Mycotoxins in Food and Feed)

Download PDF Full-Text [304 KB, uploaded 6 November 2012 16:17 CET]

Abstract: Response surface methodology was employed to optimize the degradation conditions of AFB₁ by Rhodococcus erythropolis in liquid culture. The most important factors that influence the degradation, as identified by a two-level Plackett-Burman design with six variables, were temperature, pH, liquid volume, inoculum size, agitation speed and incubation time. Central composite design (CCD) and response surface analysis were used to further investigate the interactions between these variables and to optimize the degradation efficiency of R. erythropolis based on a second-order model. The results demonstrated that the optimal parameters were: temperature, 23.2 °C; pH, 7.17; liquid volume, 24.6 mL in 100-mL flask; inoculum size, 10%; agitation speed, 180 rpm; and incubation time, 81.9 h. Under these conditions, the degradation efficiency of R. erythropolis could reach 95.8% in liquid culture, which was increased by about three times as compared to non-optimized conditions. The result by mathematic modeling has great potential for aflatoxin removal in industrial fermentation such as in food processing and ethanol production.

Keywords: Rhodococcus erythropolis; degradation efficiency; optimization; Plackett–Burman design; central composite design; response surface methodology; aflatoxins

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