Toxins 2012, 4(11), 1181-1195; doi:10.3390/toxins4111181
Article

Mathematic Modeling for Optimum Conditions on Aflatoxin B1 Degradation by the Aerobic Bacterium Rhodococcus erythropolis

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Received: 25 July 2012; in revised form: 8 October 2012 / Accepted: 31 October 2012 / Published: 6 November 2012
(This article belongs to the Special Issue Mycotoxins in Food and Feed)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Response surface methodology was employed to optimize the degradation conditions of AFB1 by Rhodococcus erythropolis in liquid culture. The most important factors that influence the degradation, as identified by a two-level Plackett-Burman design with six variables, were temperature, pH, liquid volume, inoculum size, agitation speed and incubation time. Central composite design (CCD) and response surface analysis were used to further investigate the interactions between these variables and to optimize the degradation efficiency of R. erythropolis based on a second-order model. The results demonstrated that the optimal parameters were: temperature, 23.2 °C; pH, 7.17; liquid volume, 24.6 mL in 100-mL flask; inoculum size, 10%; agitation speed, 180 rpm; and incubation time, 81.9 h. Under these conditions, the degradation efficiency of R. erythropolis could reach 95.8% in liquid culture, which was increased by about three times as compared to non-optimized conditions. The result by mathematic modeling has great potential for aflatoxin removal in industrial fermentation such as in food processing and ethanol production.
Keywords: Rhodococcus erythropolis; degradation efficiency; optimization; Plackett–Burman design; central composite design; response surface methodology; aflatoxins
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MDPI and ACS Style

Kong, Q.; Zhai, C.; Guan, B.; Li, C.; Shan, S.; Yu, J. Mathematic Modeling for Optimum Conditions on Aflatoxin B1 Degradation by the Aerobic Bacterium Rhodococcus erythropolis. Toxins 2012, 4, 1181-1195.

AMA Style

Kong Q, Zhai C, Guan B, Li C, Shan S, Yu J. Mathematic Modeling for Optimum Conditions on Aflatoxin B1 Degradation by the Aerobic Bacterium Rhodococcus erythropolis. Toxins. 2012; 4(11):1181-1195.

Chicago/Turabian Style

Kong, Qing; Zhai, Cuiping; Guan, Bin; Li, Chunjuan; Shan, Shihua; Yu, Jiujiang. 2012. "Mathematic Modeling for Optimum Conditions on Aflatoxin B1 Degradation by the Aerobic Bacterium Rhodococcus erythropolis." Toxins 4, no. 11: 1181-1195.


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