Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Toxins 2011, 3(4), 398-408; doi:10.3390/toxins3040398
Article

Detection of Ricin Contamination in Ground Beef by Electrochemiluminescence Immunosorbent Assay

Received: 16 March 2011; in revised form: 31 March 2011 / Accepted: 31 March 2011 / Published: 4 April 2011
(This article belongs to the Special Issue Ricin Toxin)
View Full-Text   |   Download PDF [183 KB, uploaded 4 April 2011]   |   Browse Figures
Abstract: Ricin is a highly toxic protein present in the seeds of Ricinus communis (castor), grown principally as a source of high quality industrial lubricant and as an ornamental. Because ricin has been used for intentional poisoning in the past and could be used to contaminate food, there is a need for analytical methodology to detect ricin in food matrices. A monoclonal antibody-based method was developed for detecting and quantifying ricin in ground beef, a complex, fatty matrix. The limit of detection was 0.5 ng/g for the electrochemiluminescence (ECL) method and 1.5 ng/g for enzyme-linked immunosorbent assay (ELISA). The detection of nanogram per gram quantities of ricin spiked into retail samples of ground beef provides approximately 10,000-fold greater sensitivity than required to detect a toxic dose of ricin (>1 mg) in a 100 g sample.
Keywords: ricin; Ricinus communis agglutinin; castor; monoclonal antibody; biothreat; electrochemiluminescence ricin; Ricinus communis agglutinin; castor; monoclonal antibody; biothreat; electrochemiluminescence
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Brandon, D.L. Detection of Ricin Contamination in Ground Beef by Electrochemiluminescence Immunosorbent Assay. Toxins 2011, 3, 398-408.

AMA Style

Brandon DL. Detection of Ricin Contamination in Ground Beef by Electrochemiluminescence Immunosorbent Assay. Toxins. 2011; 3(4):398-408.

Chicago/Turabian Style

Brandon, David L. 2011. "Detection of Ricin Contamination in Ground Beef by Electrochemiluminescence Immunosorbent Assay." Toxins 3, no. 4: 398-408.



Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert