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Toxins 2018, 10(6), 250; https://doi.org/10.3390/toxins10060250

Tb II-I, a Fraction Isolated from Tityus bahiensis Scorpion Venom, Alters Cytokines’: Level and Induces Seizures When Intrahippocampally Injected in Rats

1
Laboratory of Pharmacology, Butantan Institute, Av. Dr. Vital Brazil 1500, São Paulo 05503-900, Brazil
2
Laboratory of Biochemistry and Biophysics, Butantan Institute, Av. Dr. Vital Brazil 1500, São Paulo 05503-900, Brazil
3
Laboratory of Immunochemistry, Butantan Institute, Av. Dr. Vital Brazil 1500, São Paulo 05503-900, Brazil
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 27 April 2018 / Revised: 12 June 2018 / Accepted: 15 June 2018 / Published: 19 June 2018
(This article belongs to the Special Issue Scorpion Toxins)
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Abstract

Scorpion venoms are composed of several substances with different pharmacological activities. Neurotoxins exert their effects by targeting ion channels resulting in toxic effects to mammals, insects and crustaceans. Tb II-I, a fraction isolated from Tityus bahiensis scorpion venom, was investigated for its ability to induce neurological and immune-inflammatory effects. Two putative β-sodium channel toxins were identified in this fraction, Tb2 II and Tb 4, the latter having been completely sequenced by mass spectrometry. Male Wistar rats, stereotaxically implanted with intrahippocampal cannulas and electrodes, were injected with Tb II-I (2 µg/2 µL) via the intrahippocampal route. The behavior, electrographic activity and cellular integrity of the animals were analyzed and the intracerebral level of cytokines determined. Tb II-I injection induced seizures and damage in the hippocampus. These alterations were correlated with the changes in the level of the cytokines tumoral necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Therefore, the binding of Tb II-I to its target in the central nervous system may induce inflammation resulting in neuropathological and behavioral alterations. View Full-Text
Keywords: scorpion venom; neurotoxins; hippocampus; behavior; cytokines scorpion venom; neurotoxins; hippocampus; behavior; cytokines
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Beraldo Neto, E.; Mariano, D.O.C.; Freitas, L.A.; Dorce, A.L.C.; Martins, A.N.; Pimenta, D.C.; Portaro, F.C.V.; Cajado-Carvalho, D.; Dorce, V.A.C.; Nencioni, A.L.A. Tb II-I, a Fraction Isolated from Tityus bahiensis Scorpion Venom, Alters Cytokines’: Level and Induces Seizures When Intrahippocampally Injected in Rats. Toxins 2018, 10, 250.

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