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Toxins 2018, 10(9), 339; https://doi.org/10.3390/toxins10090339

Subtype Specificity of β-Toxin Tf1a from Tityus fasciolatus in Voltage Gated Sodium Channels

1
Laboratório de Neurofarmacologia, Departamento de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, Distrito Federal, Brazil
2
Departamento de Ciências da Saúde, Centro Universitário UDF, Brasília 70390-045, Distrito Federal, Brazil
3
Toxicology and Pharmacology, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven (KU Leuven), P.O. Box 922, Herestraat 49, 3000 Leuven, Belgium
*
Author to whom correspondence should be addressed.
Received: 13 July 2018 / Revised: 10 August 2018 / Accepted: 16 August 2018 / Published: 22 August 2018
(This article belongs to the Special Issue Scorpion Toxins)
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Abstract

Scorpion venoms are a complex mixture of components. Among them the most important are peptides, which presents the capacity to interact and modulate several ion channel subtypes, including voltage-gated sodium channels (NaV). Screening the activity of scorpion toxins on different subtypes of NaV reveals the scope of modulatory activity and, in most cases, low channel selectivity. Until now there are approximately 60 scorpion toxins experimentally assayed on NaV channels. However, the molecular bases of interaction between scorpion toxins and NaV channels are not fully elucidated. The activity description of new scorpion toxins is crucial to enhance the predictive strength of the structural–function correlations of these NaV modulatory molecules. In the present work a new scorpion toxin (Tf1a) was purified from Tityus fasciolatus venom by RP-HPLC, and characterized using electrophysiological experiments on different types of voltage-gated sodium channels. Tf1a was able to modify the normal function of NaV tested, showing to be a typical β-NaScTx. Tf1a also demonstrated an unusual capability to alter the kinetics of NaV1.5. View Full-Text
Keywords: ion channel; Na+-channel modulator; neurotoxin; scorpion; Tityus fasciolatus; venom; voltage-gated sodium channels; β-scorpion toxin ion channel; Na+-channel modulator; neurotoxin; scorpion; Tityus fasciolatus; venom; voltage-gated sodium channels; β-scorpion toxin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Mata, D.O.; Tibery, D.V.; Campos, L.A.; Camargos, T.S.; Peigneur, S.; Tytgat, J.; Schwartz, E.F. Subtype Specificity of β-Toxin Tf1a from Tityus fasciolatus in Voltage Gated Sodium Channels. Toxins 2018, 10, 339.

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