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Toxins 2009, 1(2), 196-207; doi:10.3390/toxins1020196

Fluorescence Polarization Immunoassay of Mycotoxins: A Review

Bacterial Foodborne Pathogens and Mycology Research Unit, US Department of Agriculture, Agricultural Research Service, 1815 N. University St., Peoria, IL 61604, USA
Received: 17 November 2009 / Revised: 4 December 2009 / Accepted: 9 December 2009 / Published: 10 December 2009
(This article belongs to the Special Issue Advances in Mycotoxin Research)
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Abstract

Immunoassays are routinely used in the screening of commodities and foods for fungal toxins (mycotoxins). Demands to increase speed and lower costs have lead to continued improvements in such assays. Because many reported mycotoxins are low molecular weight (below 1 kDa), immunoassays for their detection have generally been constructed in competitive heterogeneous formats. An exception is fluorescence polarization immunoassay (FPIA), a homogeneous format that does not require the separation of bound and free labels (tracer). The potential for rapid, solution phase, immunoassays has been realized in the development of FPIA for many of the major groups of mycotoxins, including aflatoxins, fumonisins, group B trichothecenes (primarily deoxynivalenol), ochratoxin A, and zearalenone. This review describes the basic principles of FPIA and summarizes recent research in this area with regard to mycotoxins. View Full-Text
Keywords: fluorescence polarization immunoassay; mycotoxins; review fluorescence polarization immunoassay; mycotoxins; review
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Maragos, C. Fluorescence Polarization Immunoassay of Mycotoxins: A Review. Toxins 2009, 1, 196-207.

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