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Toxins 2009, 1(1), 25-36; doi:10.3390/toxins1010025

Oxazolone-Induced Delayed Type Hypersensitivity Reaction in the Adult Yucatan Pigs. A Useful Model for Drug Development and Validation

1
Division of Rheumatology and Immunology, University of Virginia, USA
2
Bio-Quant, Inc., San Diego, CA, USA
3
Department of Physiology, Faculty of Medicine, University of Oslo, Norway
*
Authors to whom correspondence should be addressed.
Received: 29 July 2009 / Revised: 19 August 2009 / Accepted: 21 August 2009 / Published: 21 August 2009
(This article belongs to the Special Issue Feature Papers)
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Abstract

The purpose of this study was to establish a model of delayed type hypersensitivity (DTH) reaction in the ear skin of large animals such as adult Yucatan pigs, which may aid in evaluating the efficacy of therapeutic modalities of newly developed anti-inflammatory drugs. The pigs were sensitized with oxazolone, re-challenged with the same irritant six days later, and dosed with either vehicle or with cyclosporine A (CsA) before and after challenge. CsA reduced the redness, inhibited the accumulation of ear fluid and inflammatory cells, as well as the release of the inflammatory mediators. Further, CsA inhibited the proliferation of T cells collected from the spleens or PBMCs of CsA-treated pigs when these cells were stimulated in vitro with PMA plus Ionomycin. These results indicate that pig skin can be used to evaluate modalities for the purpose of developing drugs that may be used to treat DTH in humans.
Keywords: delayed type hypersensitivity; pigs; cyclosporine A; C-reactive protein; phorbol myristate acetate; ionomycin; chemokines; cytokines delayed type hypersensitivity; pigs; cyclosporine A; C-reactive protein; phorbol myristate acetate; ionomycin; chemokines; cytokines
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Nuhaily, S.; Damaj, B.B.; Maghazachi, A.A. Oxazolone-Induced Delayed Type Hypersensitivity Reaction in the Adult Yucatan Pigs. A Useful Model for Drug Development and Validation. Toxins 2009, 1, 25-36.

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