Toxins 2009, 1(1), 25-36; doi:10.3390/toxins1010025
Article

Oxazolone-Induced Delayed Type Hypersensitivity Reaction in the Adult Yucatan Pigs. A Useful Model for Drug Development and Validation

1 Division of Rheumatology and Immunology, University of Virginia, USA 2 Bio-Quant, Inc., San Diego, CA, USA 3 Department of Physiology, Faculty of Medicine, University of Oslo, Norway
* Authors to whom correspondence should be addressed.
Received: 29 July 2009; in revised form: 19 August 2009 / Accepted: 21 August 2009 / Published: 21 August 2009
(This article belongs to the Special Issue Feature Papers)
PDF Full-text Download PDF Full-Text [368 KB, uploaded 20 August 2009 23:36 CEST]
Abstract: The purpose of this study was to establish a model of delayed type hypersensitivity (DTH) reaction in the ear skin of large animals such as adult Yucatan pigs, which may aid in evaluating the efficacy of therapeutic modalities of newly developed anti-inflammatory drugs. The pigs were sensitized with oxazolone, re-challenged with the same irritant six days later, and dosed with either vehicle or with cyclosporine A (CsA) before and after challenge. CsA reduced the redness, inhibited the accumulation of ear fluid and inflammatory cells, as well as the release of the inflammatory mediators. Further, CsA inhibited the proliferation of T cells collected from the spleens or PBMCs of CsA-treated pigs when these cells were stimulated in vitro with PMA plus Ionomycin. These results indicate that pig skin can be used to evaluate modalities for the purpose of developing drugs that may be used to treat DTH in humans.
Keywords: delayed type hypersensitivity; pigs; cyclosporine A; C-reactive protein; phorbol myristate acetate; ionomycin; chemokines; cytokines

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Nuhaily, S.; Damaj, B.B.; Maghazachi, A.A. Oxazolone-Induced Delayed Type Hypersensitivity Reaction in the Adult Yucatan Pigs. A Useful Model for Drug Development and Validation. Toxins 2009, 1, 25-36.

AMA Style

Nuhaily S, Damaj BB, Maghazachi AA. Oxazolone-Induced Delayed Type Hypersensitivity Reaction in the Adult Yucatan Pigs. A Useful Model for Drug Development and Validation. Toxins. 2009; 1(1):25-36.

Chicago/Turabian Style

Nuhaily, Samer; Damaj, Bassam B.; Maghazachi, Azzam A. 2009. "Oxazolone-Induced Delayed Type Hypersensitivity Reaction in the Adult Yucatan Pigs. A Useful Model for Drug Development and Validation." Toxins 1, no. 1: 25-36.

Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert