Nutrients 2014, 6(2), 776-789; doi:10.3390/nu6020776
Article

Characterization of Oilseed Lipids from “DHA-Producing Camelina sativa”: A New Transformed Land Plant Containing Long-Chain Omega-3 Oils

1,* email, 2email, 2email, 2email, 1email and 2email
Received: 23 December 2013; in revised form: 28 January 2014 / Accepted: 10 February 2014 / Published: 21 February 2014
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: New and sustainable sources of long-chain (LC, ≥C20) omega-3 oils containing DHA (docosahexaenoic acid, 22:6ω3) are required to meet increasing demands. The lipid content of the oilseed of a novel transgenic, DHA-producing land plant, Camelina sativa, containing microalgal genes able to produce LC omega-3 oils, contained 36% lipid by weight with triacylglycerols (TAG) as the major lipid class in hexane extracts (96% of total lipid). Subsequent chloroform-methanol (CM) extraction recovered further lipid (~50% polar lipid, comprising glycolipids and phospholipids) and residual TAG. The main phospholipid species were phosphatidyl choline and phosphatidyl ethanolamine. The % DHA was: 6.8% (of total fatty acids) in the TAG-rich hexane extract and 4.2% in the polar lipid-rich CM extract. The relative level of ALA (α-linolenic acid, 18:3ω3) in DHA-camelina seed was higher than the control. Major sterols in both DHA- and control camelina seeds were: sitosterol, campesterol, cholesterol, brassicasterol and isofucosterol. C16–C22 fatty alcohols, including iso-branched and odd-chain alcohols were present, including high levels of iso-17:0, 17:0 and 19:0. Other alcohols present were: 16:0, iso-18:0, 18:0 and 18:1 and the proportions varied between the hexane and CM extracts. These iso-branched odd-chain fatty alcohols, to our knowledge, have not been previously reported. These components may be derived from wax esters, or free fatty alcohols.
Keywords: DHA; docosahexaenoic acid; Camelina sativa; omega-3; long-chain PUFA; triacylglycerol; polar lipids; glycolipids; phospholipids; fish oils
PDF Full-text Download PDF Full-Text [210 KB, Updated Version, uploaded 24 February 2014 09:03 CET]
The original version is still available [212 KB, uploaded 21 February 2014 13:32 CET]

Export to BibTeX |
EndNote


MDPI and ACS Style

Mansour, M.P.; Shrestha, P.; Belide, S.; Petrie, J.R.; Nichols, P.D.; Singh, S.P. Characterization of Oilseed Lipids from “DHA-Producing Camelina sativa”: A New Transformed Land Plant Containing Long-Chain Omega-3 Oils. Nutrients 2014, 6, 776-789.

AMA Style

Mansour MP, Shrestha P, Belide S, Petrie JR, Nichols PD, Singh SP. Characterization of Oilseed Lipids from “DHA-Producing Camelina sativa”: A New Transformed Land Plant Containing Long-Chain Omega-3 Oils. Nutrients. 2014; 6(2):776-789.

Chicago/Turabian Style

Mansour, Maged P.; Shrestha, Pushkar; Belide, Srinivas; Petrie, James R.; Nichols, Peter D.; Singh, Surinder P. 2014. "Characterization of Oilseed Lipids from “DHA-Producing Camelina sativa”: A New Transformed Land Plant Containing Long-Chain Omega-3 Oils." Nutrients 6, no. 2: 776-789.

Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert