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Nutrients 2014, 6(10), 4200-4217; doi:10.3390/nu6104200

Protein Content and Methyl Donors in Maternal Diet Interact to Influence the Proliferation Rate and Cell Fate of Neural Stem Cells in Rat Hippocampus

1
INRA-University of Nantes, UMR1280, 44093 Nantes, France
2
Plateforme MicroPICell, SFR Santé, 44093 Nantes, France
Present address: Elementa, 56011 Vannes, France.
*
Author to whom correspondence should be addressed.
Received: 28 July 2014 / Revised: 22 September 2014 / Accepted: 24 September 2014 / Published: 14 October 2014
(This article belongs to the Special Issue Nutritional Epigenetics)
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Abstract

Maternal diet during pregnancy and early postnatal life influences the setting up of normal physiological functions in the offspring. Epigenetic mechanisms regulate cell differentiation during embryonic development and may mediate gene/environment interactions. We showed here that high methyl donors associated with normal protein content in maternal diet increased the in vitro proliferation rate of neural stem/progenitor cells isolated from rat E19 fetuses. Gene expression on whole hippocampi at weaning confirmed this effect as evidenced by the higher expression of the Nestin and Igf2 genes, suggesting a higher amount of undifferentiated precursor cells. Additionally, protein restriction reduced the expression of the insulin receptor gene, which is essential to the action of IGFII. Inhibition of DNA methylation in neural stem/progenitor cells in vitro increased the expression of the astrocyte-specific Gfap gene and decreased the expression of the neuron-specific Dcx gene, suggesting an impact on cell differentiation. Our data suggest a complex interaction between methyl donors and protein content in maternal diet that influence the expression of major growth factors and their receptors and therefore impact the proliferation and differentiation capacities of neural stem cells, either through external hormone signals or internal genomic regulation. View Full-Text
Keywords: neurogenesis; neural stem cells; nutritional programming; methyl donors; epigenetics; hippocampus; insulin; Igf2 neurogenesis; neural stem cells; nutritional programming; methyl donors; epigenetics; hippocampus; insulin; Igf2
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Amarger, V.; Lecouillard, A.; Ancellet, L.; Grit, I.; Castellano, B.; Hulin, P.; Parnet, P. Protein Content and Methyl Donors in Maternal Diet Interact to Influence the Proliferation Rate and Cell Fate of Neural Stem Cells in Rat Hippocampus. Nutrients 2014, 6, 4200-4217.

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